O'Neill E A, Hynes R H, Bender R A
Mol Gen Genet. 1985;198(2):275-8. doi: 10.1007/BF00383006.
A recombination-deficient (Rec-) strain of Caulobacter crescentus has been isolated from a collection of mutants sensitive to ultraviolet irradiation. The Rec- mutant fails to give recombinants following phi Cr30-mediated generalized transduction or following RP4-mediated conjugation. The recombination frequency in the Rec- strain is at least 5000-fold lower than in the wild type strains. The Rec- mutant is indistinguishable from wild type in terms of morphology, growth rate, viability, and phage sensitivities, differing only in properties known to be associated with recA-type mutations in other organisms: recombination frequency, ultraviolet sensitivity, and Weigle reactivation. The map location of the rec-526 allele has not been identified, but rec-526 can be cotransferred with the fla-169 mutation by RP4-mediated conjugation at low frequency. This apparent linkage has been used to move the rec mutation to other strains. The Rec- mutant resembles recA strains of other organisms and provides a healthy strain severely deficient in recombination for use in complementation and cloning studies involving C. crescentus.
从对紫外线照射敏感的突变体库中分离出了一株新月柄杆菌重组缺陷型（Rec-）菌株。Rec-突变体在phi Cr30介导的普遍性转导或RP4介导的接合后均无法产生重组体。Rec-菌株中的重组频率比野生型菌株至少低5000倍。Rec-突变体在形态、生长速率、活力和噬菌体敏感性方面与野生型无差异，仅在已知与其他生物体中recA类型突变相关的特性上有所不同：重组频率、紫外线敏感性和韦格勒再活化。rec-526等位基因的图谱位置尚未确定，但rec-526可通过RP4介导的接合以低频率与fla-169突变共转移。这种明显的连锁关系已被用于将rec突变转移到其他菌株。Rec-突变体类似于其他生物体的recA菌株，并为涉及新月柄杆菌的互补和克隆研究提供了一株严重缺乏重组能力的健康菌株。
