Barrett J T, Rhodes C S, Ferber D M, Jenkins B, Kuhl S A, Ely B
J Bacteriol. 1982 Mar;149(3):889-96. doi: 10.1128/jb.149.3.889-896.1982.
RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid recombinants are recovered at frequencies of 10(-6) and 10(-7) per donor cell. The data from five-factor crosses were subjected to computer-assisted crossover analyses as a rapid method to determine marker order. Using this information and data from additional two- and three-factor crosses mediated by RP4 or the generalized transducing bacteriophage phi Cr30, we constructed the first genetic map for C. crescentus.
RP4介导的接合作用已被用于在新月柄杆菌中转移大片段染色体物质。在这个系统中，接合作用从多个起始点开始，每个供体细胞产生单倍体重组体的频率为10^(-6)和10^(-7)。对五因子杂交的数据进行计算机辅助交叉分析，作为确定标记顺序的快速方法。利用这些信息以及由RP4或广义转导噬菌体phi Cr30介导的另外两因子和三因子杂交的数据，我们构建了新月柄杆菌的第一张遗传图谱。
