Safarkhani Moein, Farasati Far Bahareh, Kim Su-Hyeon, Makvandi Pooyan, Park Mi-Kyung, Huh YunSuk, Rabiee Navid
Department of Biological Sciences and Bioengineering, Nano Bio High-Tech Materials Research Center, Inha University, Incheon 22212, Republic of Korea.
Department of Chemistry, Iran University of Science and Technology, Tehran 16844, Iran.
ACS Biomater Sci Eng. 2024 Apr 3. doi: 10.1021/acsbiomaterials.3c01689.
The groundbreaking gene-editing mechanism, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), paired with the protein Cas9, has significantly advanced the realms of biology, medicine, and agriculture. Through its precision in modifying genetic sequences, CRISPR holds the potential to alter the trajectory of genetic disorders and accelerate advancements in agriculture. While its therapeutic potential is profound, the technology also invites ethical debates centered on responsible use and equity in access. Parallelly, in the environmental monitoring sphere and sensing in water, especially biosensors have been instrumental in evaluating natural water sources' quality. These biosensors, integrating biological components with detection techniques, have the potential to revolutionize healthcare by providing rapid and minimally invasive diagnostic methods. However, the design and application of these sensors bring forth challenges, especially in ensuring sensitivity, selectivity, and ethical data handling. This article delves into the prospective use of CRISPR-Cas technology for sensing in water, exploring its capabilities in detecting diverse biomarkers, hazardous substances, and varied reactions in water and wastewater systems.
开创性的基因编辑机制CRISPR（成簇规律间隔短回文重复序列）与蛋白质Cas9相结合，极大地推动了生物学、医学和农业领域的发展。通过其在修改基因序列方面的精准性，CRISPR有潜力改变遗传疾病的发展轨迹，并加速农业进步。虽然其治疗潜力巨大，但这项技术也引发了围绕负责任使用和获取公平性的伦理辩论。与此同时，在环境监测领域以及水的传感方面，尤其是生物传感器在评估天然水源质量方面发挥了重要作用。这些生物传感器将生物成分与检测技术相结合，有可能通过提供快速且微创的诊断方法来彻底改变医疗保健。然而，这些传感器的设计和应用带来了挑战，尤其是在确保灵敏度、选择性和道德数据处理方面。本文深入探讨了CRISPR-Cas技术在水中传感的潜在用途，探索其在检测水和废水系统中的各种生物标志物、有害物质及不同反应方面的能力。
