循环 microRNA-3552 作为大鼠缺血性脑卒中的潜在生物标志物。
Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats.
机构信息
Department of Neurology, Hebei General Hospital, No. 348, Heping West Road, Shijiazhuang, 050050 Hebei Province, China.
出版信息
Biomed Res Int. 2020 Jul 16;2020:4501393. doi: 10.1155/2020/4501393. eCollection 2020.
OBJECTIVE
With the growing incidence of ischemic stroke worldwide, there is an urgent need to identify blood biomarkers for ischemic stroke patients. Thus, our aim was to identify potential circulating microRNA (miRNA) as a potential biomarker and to explore its potential mechanism for ischemic stroke in rats.
METHODS
The mRNA dataset GSE97537 and miRNA dataset GSE97532 were downloaded from the Gene Expression Omnibus (GEO) GSE97537 including 7 middle cerebral artery occlusion (MCAO) rat brain tissues and 5 sham-operated rat brain tissues GSE97532 including 6 MCAO rat blood samples and 3 sham-operated rat blood samples. Differentially expressed mRNAs and miRNAs with corrected value ≤ 0.01 and fold change ≥2 or ≤0.05 were identified. To explore potential biological processes and pathways of differentially expressed mRNAs, functional enrichment analysis was performed. The target mRNAs of differentially expressed miRNAs were predicted using DNA Intelligent Analysis (DIANA)-microT tools. The target mRNAs and differentially expressed mRNAs were intersected.
RESULTS
1228 differentially expressed mRNAs in MCAO rat brain tissues were identified. Highly expressed mRNAs were mainly enriched in the inflammatory responses. Nine differentially expressed miRNAs were identified in MCAO rat blood samples. A total of 673 target mRNAs were predicted to significantly bind these differentially expressed miRNAs. Among them, 54 target mRNAs were differentially expressed in MCAO rat blood samples. Enrichment analysis results showed that these 54 target mRNAs were closely related to neurological diseases and immune responses. Among all miRNA-mRNA relationship, miR-3552-CASP3 interaction was identified, indicating that CASP3 might be mediated by miR-3552. Functional enrichment analysis revealed that CASP3 was involved in the apoptosis pathway, indicating that miR-3552 might participate in apoptosis by CASP3.
CONCLUSION
Our findings reveal that circulating miR-3552 shows promise as a potential biomarker for ischemic stroke in rats.
目的
随着全球缺血性中风发病率的上升，迫切需要确定缺血性中风患者的血液生物标志物。因此，我们的目的是确定潜在的循环 microRNA（miRNA）作为一种潜在的生物标志物，并探讨其在大鼠缺血性中风中的潜在机制。
方法
从基因表达综合数据库（GEO）下载 mRNA 数据集 GSE97537 和 miRNA 数据集 GSE97532，包括 7 个大脑中动脉闭塞（MCAO）大鼠脑组织和 5 个假手术大鼠脑组织 GSE97532 包括 6 个 MCAO 大鼠血液样本和 3 个假手术大鼠血液样本。鉴定校正值 ≤ 0.01 且倍数变化≥2 或≤0.05 的差异表达 mRNA 和 miRNA。为了探讨差异表达 mRNA 的潜在生物学过程和途径，进行了功能富集分析。使用 DNA 智能分析（DIANA）-microT 工具预测差异表达 miRNA 的靶 mRNA。将差异表达的 miRNA 的靶 mRNA 与差异表达的 mRNA 进行交集。
结果
在 MCAO 大鼠脑组织中鉴定出 1228 个差异表达的 mRNA。高表达的 mRNA 主要富集在炎症反应中。在 MCAO 大鼠血液样本中鉴定出 9 个差异表达的 miRNA。总共预测了 673 个靶 mRNA 与这些差异表达的 miRNA 显著结合。其中，在 MCAO 大鼠血液样本中 54 个靶 mRNA 差异表达。富集分析结果表明，这些 54 个靶 mRNA 与神经疾病和免疫反应密切相关。在所有 miRNA-mRNA 关系中，鉴定到 miR-3552-CASP3 相互作用，表明 CASP3 可能受 miR-3552 介导。功能富集分析表明，CASP3 参与了凋亡途径，表明 miR-3552 可能通过 CASP3 参与凋亡。
结论
我们的研究结果表明，循环 miR-3552 有望成为大鼠缺血性中风的潜在生物标志物。
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