用荧光交联剂稳定肌球蛋白亚片段1中的初级环。
Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.
作者信息
Mornet D, Ue K, Morales M F
出版信息
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1658-62. doi: 10.1073/pnas.82.6.1658.
Abstract
A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain and seems to place the dibromobimane near the ATP-perturbable tryptophan.
摘要
一种双功能荧光烷基化试剂——二溴双马来酰亚胺，已被用于稳定肌球蛋白亚片段1（S-1）中预先存在的一级环。所实现的交联将20 kDa结构域（以前称为“20K”结构域）的半胱氨酸-707（称为巯基“SH1”）与50 kDa结构域的一个硫醇连接起来，并且似乎将二溴双马来酰亚胺置于靠近ATP可干扰色氨酸的位置。
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