Brennecke S P, Castle B M, Demers L M, Turnbull A C
Br J Obstet Gynaecol. 1985 Apr;92(4):345-9. doi: 10.1111/j.1471-0528.1985.tb01107.x.
Because of methodological problems associated with the measurement in biological fluids of both prostaglandin E2 (PGE2) and its unstable principal circulating metabolite 13,14-dihydro-15-keto-PGE2 (PGEM), there is little reliable information on these prostaglandins in human pregnancy and parturition. The recent discovery of a stable PGEM degradation product 11-deoxy-13,14-dihydro-15-keto-11 beta, 16 epsilon-cyclo-PGE2 (bicyclo-PGEM) has provided a means of studying endogenous plasma levels of PGEM which circumvents the problems encountered with direct measurements of PGE2 and PGEM. Using a radioimmunoassay for bicyclo-PGEM we have therefore determined maternal peripheral plasma PGE2 metabolite levels during human gestation. PGE2 metabolite levels did not alter significantly during the second or third trimesters nor during labour. This contrasts with maternal peripheral plasma levels of the principal circulating metabolite of PGF2 alpha 13,14-dihydro-15-keto-PGF2 alpha (PGFM) which increases several fold during labour. Compared to PGE2 therefore, PGF2 alpha may be quantitatively the more significant prostaglandin associated with human parturition.
由于在生物体液中测量前列腺素E2（PGE2）及其不稳定的主要循环代谢物13,14-二氢-15-酮-PGE2（PGEM）存在方法学问题，关于这些前列腺素在人类妊娠和分娩中的可靠信息很少。最近发现了一种稳定的PGEM降解产物11-脱氧-13,14-二氢-15-酮-11β,16ε-环-PGE2（双环-PGEM），这提供了一种研究PGEM内源性血浆水平的方法，避免了直接测量PGE2和PGEM时遇到的问题。因此，我们使用双环-PGEM放射免疫分析法测定了人类妊娠期间母体外周血浆PGE2代谢物水平。在妊娠中期或晚期以及分娩期间，PGE2代谢物水平没有显著变化。这与PGF2α的主要循环代谢物13,14-二氢-15-酮-PGF2α（PGFM）的母体外周血浆水平形成对比，PGFM在分娩期间会增加几倍。因此，与PGE2相比，PGF2α在数量上可能是与人类分娩相关的更重要的前列腺素。
