Cheng G Y, Minden M D, Toyonaga B, Mak T W, McCulloch E A
J Exp Med. 1986 Feb 1;163(2):414-24. doi: 10.1084/jem.163.2.414.
The organization and expression of the beta chain of T cell antigen receptor gene (beta-TCR) and Ig H and L chain genes were analyzed by Southern blot technique in 24 patients with a diagnosis of acute myeloblastic leukemia (AML). Rearrangements of the beta-TCR genes were seen in DNA samples from 3 of the 24 patients. One of these three patients also showed rearrangement of the Ig H chain gene. RNA samples from all three patients expressed a beta-TCR gene transcript on dot blot analysis. However, on Northern blot analysis, one patient expressed an incomplete 1.0 kb transcript and no Ig H chain mRNA, despite a rearranged configuration. The karyotypes of two of these patients showed abnormalities involving chromosome 7. Rearrangements of T cell antigen receptor genes may occur in nonlymphoid malignancy, and is consistent with the concept of lineage infidelity in AML.
采用Southern印迹技术分析了24例急性髓细胞白血病（AML）患者T细胞抗原受体基因β链（β-TCR）以及Ig H和L链基因的组织和表达情况。在24例患者的DNA样本中，有3例出现了β-TCR基因重排。这3例患者中的1例还显示出Ig H链基因重排。在斑点印迹分析中，所有3例患者的RNA样本均表达β-TCR基因转录本。然而，在Northern印迹分析中，1例患者尽管基因构型重排，但仅表达了一条不完整的1.0 kb转录本，未检测到Ig H链mRNA。其中2例患者的核型显示涉及7号染色体的异常。T细胞抗原受体基因重排可能发生于非淋巴系统恶性肿瘤，这与AML中谱系不忠实的概念相符。
