Ren Jing, Li Yumei, Ni Hongyu, Zhang Yan, Zhao Puze, Xiao Qingxing, Hong Xiaoqing, Zhang Ziyi, Yin Yijing, Li Xiaohui, Zhang Yonghong, Yang Yuwei
College of Animal Science, Jilin University, Changchun 130062, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, China.
Anim Biosci. 2024 Aug;37(8):1428-1439. doi: 10.5713/ab.23.0475. Epub 2024 Apr 1.
Compared to Mimas pigeons, Shiqi pigeons exhibit greater tolerance to coarse feeding because of their abundant gut microbiota. Here, to investigate the potential of utilizing intestinal flora derived from Shiqi pigeons, the intestinal flora and body indices of Mimas squabs were evaluated after fecal microbiota transplantation (FMT) from donors.
A total of 90 one-day-old squabs were randomly divided into the control group (CON), the low-concentration group (LC) and the high-concentration group (HC): gavaged with 200 μL of bacterial solution at concentrations of 0, 0.1, and 0.2 g/15 mL, respectively.
The results suggested that FMT improved the body weight of Mimas squabs in the HC and LC groups (p<0.01), and 0.1 g/15 mL was the optimal dose during FMT. After 16S rRNA sequencing was performed, compared to those in the CON group, the abundance levels of microflora, especially Lactobacillus, Muribaculaceae, and Megasphaera (p<0.05), in the FMT-treated groups were markedly greater. Random forest analysis indicated that the main functions of key microbes involve pathways associated with metabolism, further illustrating their important role in the host body.
FMT has been determined to be a viable method for augmenting the weight and intestinal microbiota of squabs, representing a unique avenue for enhancing the economic feasibility of squab breeding.
与米玛斯鸽相比，石岐鸽因其丰富的肠道微生物群而对粗放饲养表现出更强的耐受性。在此，为了研究利用石岐鸽肠道菌群的潜力，在接受来自供体的粪便微生物群移植（FMT）后，对米玛斯乳鸽的肠道菌群和身体指标进行了评估。
将90只1日龄乳鸽随机分为对照组（CON）、低浓度组（LC）和高浓度组（HC）：分别用浓度为0、0.1和0.2 g/15 mL的200 μL菌液进行灌胃。
结果表明，FMT提高了HC组和LC组米玛斯乳鸽的体重（p<0.01），且0.1 g/15 mL是FMT过程中的最佳剂量。进行16S rRNA测序后，与CON组相比，FMT处理组的微生物群落丰度水平，尤其是乳酸杆菌、毛螺菌科和巨球型菌的丰度水平显著更高（p<0.05）。随机森林分析表明，关键微生物的主要功能涉及与代谢相关的途径，进一步说明了它们在宿主体内的重要作用。
FMT已被确定为增加乳鸽体重和肠道微生物群的可行方法，是提高乳鸽养殖经济可行性的独特途径。
