Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, PR China; School of Life Sciences, Shandong Normal University, Jinan 250014, PR China; Key Laboratory of Livestock and Poultry Multi-omics of MARA, Jinan 250100, PR China.
Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, PR China; Key Laboratory of Livestock and Poultry Multi-omics of MARA, Jinan 250100, PR China.
Vet Microbiol. 2022 Jun;269:109449. doi: 10.1016/j.vetmic.2022.109449. Epub 2022 May 5.
The intestinal microbiota plays important roles in animal health and growth. We investigated the efficacy and mechanisms of fecal microbiota transplantation (FMT) from adult SPF chickens against Salmonella Enteritidis (SE) infection in chicks. We transplanted 160 recipient SPF chicks (1-day-old) that were randomly divided into four groups, Ca (challenge), Cb (non-challenge), Fa (FMT and challenge) and Fb (FMT without challenge). The experiment lasted 40 days. We found that FMT reduced mortality as well as liver inflammatory lesions, promoted weight gain, improved immunity, ameliorated the digestion and absorption ability and inhibited SE colonization in the liver of challenged chicks. 16S rRNA gene high-throughput sequencing indicated that SE challenge caused a significant increase in the relative abundance of Parasutterella in the cecal microbiota of the recipient chicks (P < 0.05). FMT led to the maturation of the intestinal flora of recipients and the relative abundance of the Bacteroides, Rikenellaceae_ RC9_ gut_ group, Prevotellaceae_ UCG_ 001, Prevotellaceae_ Ga6A1_ group and Parabacteroides was significantly increased (P < 0.05). FMT from adult SPF chickens regulated the intestinal microbiota of chicks and increased resistance to SE infection.
肠道微生物群在动物健康和生长中发挥着重要作用。我们研究了来自 SPF 成年鸡的粪便微生物群移植（FMT）对雏鸡感染肠炎沙门氏菌（SE）的功效和机制。我们移植了 160 只接受 SPF 的雏鸡（1 日龄），这些雏鸡随机分为 4 组，Ca（挑战）、Cb（非挑战）、Fa（FMT 和挑战）和 Fb（FMT 无挑战）。实验持续了 40 天。我们发现 FMT 降低了死亡率和肝脏炎症病变，促进了体重增加，提高了免疫力，改善了消化吸收能力，并抑制了 SE 在受挑战雏鸡肝脏中的定植。16S rRNA 基因高通量测序表明，SE 挑战导致受者盲肠微生物群中 Parasutterella 的相对丰度显著增加（P < 0.05）。FMT 导致受者肠道菌群的成熟，Bacteroides、Rikenellaceae_RC9_肠道_群、Prevotellaceae_UCG_001、Prevotellaceae_Ga6A1_群和 Parabacteroides 的相对丰度显著增加（P < 0.05）。来自 SPF 成年鸡的 FMT 调节了雏鸡的肠道微生物群，增加了对 SE 感染的抵抗力。
