Department of Medical Laboratory Science, College of Health Science, Woldia University, Woldia, Ethiopia.
Department of Medical Laboratory Science, College of Medicine and Health Science, Wollo University, Dessie, Ethiopia.
BMC Microbiol. 2024 Apr 4;24(1):116. doi: 10.1186/s12866-024-03224-5.
Antimicrobial resistant bacteria among hospitalized patients are becoming a major public health threat worldwide, mainly in developing countries. Infections by these multidrug resistant pathogens cause high rate of mortality, prolong hospital stays, and affect individual and country economies in greater amounts. Thus, this study aimed to assess the bacterial profile, antimicrobial susceptibility status, and associated factors of isolates from hospitalized patients at the Dessie Comprehensive Specialized Hospital.
This hospital-based cross-sectional study was conducted between February and April 2021. Consecutive sampling was used to select the study participants. All bacterial isolates were identified using standard bacteriological techniques. Antibiotic susceptibility testing was performed using disk diffusion technique. The data was analyzed using SPSS version 25. Descriptive statistics and logistic regression were used. A P-value of less than 0.05 was considered statistically significant.
Of 384 clinical samples (blood, urine, stool, wound, vaginal discharge, and ear discharge) processed 180 (46.9%) were culture positive. Overall, Escherichia coli was the predominant isolate (41; 22.8%), followed by Staphylococcus aureus (36; 20%). Most of the isolates were from blood (70; 38.9%). The level of overall drug resistance of the gram-negative bacteria isolates for ampicillin, tetracycline, and cotrimoxazole was (104; 88.1%), (79; 75.9%), and (78; 75.0%), respectively. The overall multidrug rate of isolates was 143 (79.4%). Variables such as history of invasive procedures, chronic underlying diseases, history of hospitalization, and habit of eating raw animal products were statistically significant for the acquisition of bacterial infection.
E. Coli and S. aureus were the most common isolates. Most of the isolates were resistant to commonly prescribed antibiotics. And also, consumption of raw animal products, chronic underlying disease, previous hospitalization, history of invasive procedures, and educational status were associated with the acquisition of bacterial infections. Therefore, routine antimicrobial susceptibility testing, proper patient management, wise use of antibiotics in clinical settings and health education are recommended.
住院患者中的抗菌药物耐药细菌正成为全球主要的公共卫生威胁，主要在发展中国家。这些多药耐药病原体引起的感染导致高死亡率、延长住院时间，并对个人和国家经济造成更大的影响。因此，本研究旨在评估德西综合专科医院住院患者分离株的细菌谱、抗菌药物敏感性状况及相关因素。
这是一项在 2021 年 2 月至 4 月期间进行的基于医院的横断面研究。采用连续抽样法选择研究对象。所有细菌分离株均采用标准细菌学技术鉴定。采用纸片扩散法进行抗菌药物敏感性试验。采用 SPSS 版本 25 进行数据分析。采用描述性统计和逻辑回归进行分析。P 值小于 0.05 被认为具有统计学意义。
对处理的 384 份临床样本（血液、尿液、粪便、伤口、阴道分泌物和耳分泌物）进行培养，有 180 份（46.9%）为培养阳性。总的来说，大肠杆菌是主要的分离株（41 株；22.8%），其次是金黄色葡萄球菌（36 株；20%）。大多数分离株来自血液（70 株；38.9%）。革兰氏阴性菌分离株对氨苄西林、四环素和复方磺胺甲噁唑的总体耐药率分别为（104 株；88.1%）、（79 株；75.9%）和（78 株；75.0%）。分离株的总体多重耐药率为 143 株（79.4%）。有创操作史、慢性基础疾病、住院史和食用生动物产品的习惯等变量对细菌感染的获得具有统计学意义。
大肠杆菌和金黄色葡萄球菌是最常见的分离株。大多数分离株对常用抗生素有耐药性。此外，食用生动物产品、慢性基础疾病、既往住院史、有创操作史和教育程度与细菌感染的获得有关。因此，建议常规进行抗菌药物敏感性试验、适当的患者管理、在临床环境中明智地使用抗生素和开展健康教育。
