Oral Center, Fujian Provincial Governmental Hospital, Fuzhou, China.
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
BMC Oral Health. 2024 Apr 4;24(1):413. doi: 10.1186/s12903-024-04197-7.
Tissue engineering has attracted recent attention as a promising bone repair and reconstruction approach. Dental pulp stem cells (DPSCs) are pluripotent and can differentiate into bone cells with the correct environment and substrate. Therefore, suitable scaffold materials are essential for fabricating functional three-dimensional (3D) tissue and tissue regeneration. Composite scaffolds consisting of biodegradable polymers are very promising constructs. This study aims to verify the biological function of human DPSCs seeded onto composite scaffolds based on graphene oxide (GO) and poly-L-lactic acid (PLLA).
The surface morphology was observed under scanning electron microscopy (SEM). Chemical composition was evaluated with Fourier transform infrared (FTIR) spectroscopy. The biocompatibility of GO/PLLA scaffolds was assessed using phalloidin staining of cytoskeletal actin filaments, live/dead staining, and a CCK-8 assay. The effect of GO/PLLA scaffolds on cell osteogenic differentiation was detected through ALP staining, ALP activity assays, and alizarin red S staining, complemented by quantitative real-time PCR (qRT-PCR) analysis.
Our data showed that GO and PLLA are successfully integrated and the GO/PLLA scaffolds exhibit favorable bioactivity and biocompatibility towards DPSCs. Additionally, it was observed that the 0.15% GO/PLLA scaffold group promoted DPSC proliferation and osteogenic differentiation by forming more calcium nodules, showing a higher intensity of ALP staining and ALP activity, and enhancing the expression levels of differentiation marker genes RUNX2 and COL1.
These results demonstrate that the GO/PLLA scaffold is a feasible composite material suitable for cell culture and holds promising applications for oral bone tissue engineering.
组织工程作为一种有前途的骨修复和重建方法，近来受到关注。牙髓干细胞（DPSCs）具有多能性，在适当的环境和基质下可分化为成骨细胞。因此，合适的支架材料对于构建功能性三维（3D）组织和组织再生至关重要。由可生物降解聚合物组成的复合支架是非常有前途的构建体。本研究旨在验证基于氧化石墨烯（GO）和聚-L-乳酸（PLLA）的人牙髓干细胞（DPSCs）接种到复合支架上的生物学功能。
通过扫描电子显微镜（SEM）观察表面形态。用傅里叶变换红外（FTIR）光谱评估化学组成。通过鬼笔环肽染色细胞骨架肌动蛋白丝、死活染色和 CCK-8 测定评估 GO/PLLA 支架的生物相容性。通过碱性磷酸酶（ALP）染色、ALP 活性测定和茜素红 S 染色检测 GO/PLLA 支架对细胞成骨分化的影响，并通过定量实时 PCR（qRT-PCR）分析进行补充。
我们的数据表明，GO 和 PLLA 成功结合，GO/PLLA 支架对 DPSCs 具有良好的生物活性和生物相容性。此外，观察到 0.15% GO/PLLA 支架组通过形成更多的钙结节来促进 DPSC 的增殖和成骨分化，表现出更高强度的 ALP 染色和 ALP 活性，并增强分化标志物基因 RUNX2 和 COL1 的表达水平。
这些结果表明，GO/PLLA 支架是一种可行的复合材料，适合细胞培养，在口腔骨组织工程中有广阔的应用前景。
