在牛脑提取物中检测胆固醇和β-谷甾醇硫酸酯的亲脂性复合物。
Detection in extracts of bovine brain of lipophilic complexes of sulfate esters of cholesterol and beta-sitosterol.
作者信息
Prasad V V, el-Maraghy E, Ponticorvo L, Lieberman S
出版信息
Proc Natl Acad Sci U S A. 1985 May;82(9):2657-9. doi: 10.1073/pnas.82.9.2657.
Abstract
Evidence indicating that there exist in bovine brains hitherto-unrecognized lipophilic conjugates of sterol sulfates is presented. These conjugates are soluble in nonpolar solvents and, when heated in methanol containing pyridine, yield polar sterol conjugates. These polar substances have the chromatographic mobility of sterol sulfates and are cleaved to free sterols when subjected to a solvolytic process known to be specific for sulfate esters. The brain sterols that have been identified in this way are cholesterol and beta-sitosterol.
摘要
有证据表明，牛脑中存在迄今未被认识的硫酸酯类亲脂性共轭物。这些共轭物可溶于非极性溶剂，当在含吡啶的甲醇中加热时，会生成极性甾醇共轭物。这些极性物质具有硫酸甾醇的色谱迁移率，并且在经历已知对硫酸酯具有特异性的溶剂分解过程时会裂解为游离甾醇。通过这种方式鉴定出的脑甾醇为胆固醇和β-谷甾醇。
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