Harsin Akram Oftadeh, Firozian Farzin, Ahmadabadi Amir Nili, Soleimani Meysam, Ranjbar Akram
Department of Pharmaclogy and Toxicology, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, 6517838678 Iran.
Departments of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
Indian J Clin Biochem. 2024 Apr;39(2):283-290. doi: 10.1007/s12291-022-01096-y. Epub 2022 Nov 17.
Paraquat (PQ) herbicide poisoning is a severe medical problem in developing countries without suitable therapy. This study aimed to investigate the effects of crocin (CCN) and nano crocin (NCCN) on PQ -induced toxicity in the MRC-5 cell line. The results showed that the particle size of NCCN was 140.3 ± 18.0 nm, and the zeta potential of the optimal crocin-loaded niosomes was 23.4 ± 2.8 mV. The NCCN was more effective than CCN in the inhibition of PQ-induced toxicity. Treatment of the MRC-5 cells leads to a decrease in ROS and an increase in SOD, CAT, GPX, and TAC levels in PQ-CCN and PQ-NCCN groups compared with the PQ group. These changes tended to be positively associated with the NCCN compared to CCN. Overall, NCCN was more effective than crocin in treating PQ-induced toxicity in vitro and deserved further preclinical consideration.
百草枯（PQ）除草剂中毒在缺乏合适治疗方法的发展中国家是一个严重的医学问题。本研究旨在探讨藏红花素（CCN）和纳米藏红花素（NCCN）对MRC-5细胞系中PQ诱导毒性的影响。结果显示，NCCN的粒径为140.3±18.0nm，最佳载藏红花素脂质体的ζ电位为23.4±2.8mV。NCCN在抑制PQ诱导的毒性方面比CCN更有效。与PQ组相比，用PQ-CCN和PQ-NCCN处理MRC-5细胞导致ROS减少，SOD、CAT、GPX和TAC水平增加。与CCN相比，这些变化与NCCN呈正相关。总体而言，NCCN在体外治疗PQ诱导的毒性方面比藏红花素更有效，值得进一步进行临床前研究。
