Ayoka Thecla Okeahunwa, Nwachukwu Ngwu, Ene Aloysius Chinedu, Igwe Chidi Uzoma, Nnadi Charles Okeke
Department of Science Laboratory Technology (Biochemistry Unit), Faculty of Physical Sciences, University of Nigeria, Nsukka, Enugu State Nigeria.
Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Imo State Nigeria.
Indian J Clin Biochem. 2024 Apr;39(2):188-196. doi: 10.1007/s12291-022-01095-z. Epub 2022 Nov 12.
The study investigated the hepatocurative activity of the bulk alkaloids of in a tetrachloromethane (CCl)-induced hepatotoxicity model in rats. The hepatocurative activity of the alkaloids at 200, 400 and 600 mg/kg doses was demonstrated by the assay of both enzymic and non-enzymic parameters. Sections of the liver were also subjected to histological examinations. Mapping techniques and data visualization approaches were adopted in finding relationships between the enzymic and non-enzymic parameters and the treatment groups. The bulk alkaloids caused dose-dependent effects on both the enzymic and non-enzymic parameters. The bulk alkaloids elicited a significant reduction ( < 0.05) in all liver and antioxidant enzymes activities compared with the untreated. The 600 mg/kg dose caused the restoration of the ALP, ALT and AST to 76.16, 10.72 and 11.83 iU/L respectively similar to the standard butylated hydroxytoluene. The 600 mg/kg dose also caused a slight increase in the activities of SOD, catalase and GPx to 11.45. 1.37 and 11.66 iU/L respectively when compared with the untreated rats. In the non-enzymic assays, the 600 mg/kg dose elicited a significant ( < 0.05) upregulation in the total bilirubin (1.18 mg/100 mL), total protein (3.75 g/dL), HDL (1.80 mMol/L) and vitamin C (2.41 mg/dL) and decrease in the CHOL (3.35 g/dL), TAG (1.85 mMol/L), LDL (0.67 mMol/L), BUN (39.55 mg/dL) and MDA (1.13 nMol/mL) when compared with the untreated rats. The restoration of the natural histo-architecture of the CCl-damaged liver by the alkaloids further evidenced the hepatocurative activity of the bulk alkaloids.
该研究在四氯化碳（CCl）诱导的大鼠肝毒性模型中，研究了[具体物质]总生物碱的保肝活性。通过酶学和非酶学参数测定，证明了200、400和600mg/kg剂量生物碱的保肝活性。还对肝脏切片进行了组织学检查。采用映射技术和数据可视化方法来寻找酶学和非酶学参数与治疗组之间的关系。总生物碱对酶学和非酶学参数均产生剂量依赖性影响。与未处理组相比，总生物碱使所有肝脏和抗氧化酶活性显著降低（P<0.05）。600mg/kg剂量使碱性磷酸酶（ALP）、谷丙转氨酶（ALT）和谷草转氨酶（AST）分别恢复到76.16、10.72和11.83iU/L，与标准丁基羟基甲苯相似。与未处理大鼠相比，600mg/kg剂量还使超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GPx）活性分别略有增加至11.45、1.37和11.66iU/L。在非酶学测定中，与未处理大鼠相比，600mg/kg剂量使总胆红素（1.18mg/100mL）、总蛋白（3.75g/dL）、高密度脂蛋白（HDL，1.80mmol/L）和维生素C（2.41mg/dL）显著上调（P<0.05），使胆固醇（CHOL，3.35g/dL）、甘油三酯（TAG，1.85mmol/L）、低密度脂蛋白（LDL，0.67mmol/L）、血尿素氮（BUN，39.55mg/dL）和丙二醛（MDA，1.13nmol/mL）降低。生物碱对CCl损伤肝脏自然组织结构的恢复进一步证明了总生物碱的保肝活性。
