Fràter-Schröder M, Prochazka M, Haller O, Arwert F, Porck H J, Skow L C, Lundin L G, Hilkens J, Hilgers J
Biochem Genet. 1985 Feb;23(1-2):139-53. doi: 10.1007/BF00499119.
Somatic cell hybrids, recombinant inbred (RI) mouse strains, and backcross breeding experiments were used to locate the gene of transcobalamin II (Tcn-2), the vitamin B12 binding protein in mouse serum. TCN-2 was found to be useful genetic marker in the somatic cell hybrids. Selected hybrid clones were derived from fusions between GR mouse cells and the Chinese hamster cell line E36. Analysis of mouse specific chromosomal enzyme markers in relationship to TCN-2 secretion, in the hybrid clones, provided provisional evidence for assignment of the Tcn-2 locus to chromosome 11. The strain distribution pattern of the TCN-2 variants S and F in the RI series CXS, constructed from the cross of BALB/cHeA (TCN-2S) with STS/A (TCN-2F), implied a close linkage with the hemoglobin alpha-chain locus (Hba) on chromosome 11. Backcross breeding using inbred strains confirmed these findings and located the Tcn-2 gene closest to the centromere, linked with waved 2 (wa-2) and Hba with recombination frequencies of 6.9 and 19.2% each. The linkage group Tcn-2/wa-2/Hba was established.
利用体细胞杂种、重组近交（RI）小鼠品系和回交育种实验来定位小鼠血清中维生素B12结合蛋白转钴胺素II（Tcn-2）的基因。发现TCN-2在体细胞杂种中是一种有用的遗传标记。选定的杂种克隆源自GR小鼠细胞与中国仓鼠细胞系E36之间的融合。对杂种克隆中与TCN-2分泌相关的小鼠特异性染色体酶标记进行分析，为将Tcn-2基因座定位于11号染色体提供了初步证据。由BALB/cHeA（TCN-2S）与STS/A（TCN-2F）杂交构建的RI系列CXS中，TCN-2变体S和F的品系分布模式表明其与11号染色体上的血红蛋白α链基因座（Hba）紧密连锁。使用近交系进行的回交育种证实了这些发现，并将Tcn-2基因定位在最靠近着丝粒的位置，与波状毛2（wa-2）和Hba连锁，重组频率分别为6.9%和19.2%。建立了Tcn-2/wa-2/Hba连锁群。
