Kozak C A, Peyser M, Krall M, Mariano T M, Kumar C S, Pestka S, Mock B A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Genomics. 1990 Nov;8(3):519-24. doi: 10.1016/0888-7543(90)90039-w.
Somatic cell hybrids, recombinant inbred (RI) strains, and progeny of an intersubspecific backcross were typed by Southern blot analysis to prepare a linkage map of mouse chromosome 10. The seven genetic markers in this map, four of which had not previously been positioned, include genes involved in oncogenesis (Gli, Myb, Tra-1), proviral integration (Emv-25), and immune responses (Ifg, Ifgr, Pfp). The linkage map spans much of the chromosome and covers a region of the mouse genome with few molecular markers. The gene order established here demonstrates that the genes for murine interferon-gamma (Ifg) and its receptor (Ifgr) are at opposite ends of the chromosome and that Ifgr and the Myb oncogene are closely linked, a factor that may be related to their joint transcriptional enhancement in some plasmacytoid lymphosarcomas.
通过Southern印迹分析对体细胞杂种、重组近交（RI）系以及种间回交后代进行分型，以构建小鼠10号染色体的连锁图谱。该图谱中的七个遗传标记，其中四个此前未定位，包括参与肿瘤发生的基因（Gli、Myb、Tra-1）、前病毒整合基因（Emv-25）以及免疫反应相关基因（Ifg、Ifgr、Pfp）。该连锁图谱覆盖了染色体的大部分区域，涵盖了小鼠基因组中分子标记较少的一个区域。此处确定的基因顺序表明，小鼠γ干扰素（Ifg）及其受体（Ifgr）的基因位于染色体的两端，并且Ifgr与Myb癌基因紧密连锁，这一因素可能与它们在某些浆细胞样淋巴瘤中的联合转录增强有关。
