D'Eustachio P, Jadidi S, Fuhlbrigge R C, Gray P W, Chaplin D D
Department of Biochemistry, New York University Medical Center, NY 10016.
Immunogenetics. 1987;26(6):339-43. doi: 10.1007/BF00343701.
Two interleukin-1 polypeptides, alpha and beta, are known, and cDNAs corresponding to each have been described. Genomic cloning and Southern blotting experiments suggest that in the mouse each is encoded by a gene present in one copy per haploid genome. Analysis of a panel of somatic cell hybrids carrying various mouse chromosomes on a constant Chinese hamster background indicates that both genes map to mouse chromosome 2. Further, analysis of the inheritance of DNA restriction fragment length polymorphisms associated with each gene in recombinant inbred strains of mice shows the two loci to be tightly linked to one another, and to lie approximately 4.7 centimorgans distal to B2m (beta-2 microglobulin). We have named the locus encoding IL-1 alpha Il-1 alpha and the locus encoding IL-1 beta Il-1b.
已知有两种白细胞介素-1多肽，即α和β，并且已经描述了与之对应的cDNA。基因组克隆和Southern印迹实验表明，在小鼠中，每种多肽均由单倍体基因组中每个存在一个拷贝的基因编码。对一组在恒定的中国仓鼠背景上携带各种小鼠染色体的体细胞杂种进行分析表明，这两个基因均定位于小鼠2号染色体。此外，对小鼠重组近交系中与每个基因相关的DNA限制性片段长度多态性的遗传分析表明，这两个基因座彼此紧密连锁，并且位于距B2m（β-2微球蛋白）约4.7厘摩的远端。我们将编码IL-1α的基因座命名为Il-1α，将编码IL-1β的基因座命名为Il-1b。
