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腺相关病毒载体生产的质量控制。

Quality control for Adeno-associated viral vector production.

作者信息

Ersing Ina, Rego Meghan, Wang Chen, Zhang Yijun, DeMaio Kate Harten, Tillgren Michelle, Fava Alanna, Clouse Gabrielle, Patrick Marcella, Guerin Karen, Fan Melina

机构信息

Addgene, 490 Arsenal Way, Suite 100, Watertown, MA 02472, USA.

Vedere Bio II, Inc, 300 Technology square, Cambridge, MA 02139, USA.

出版信息

Neuromethods. 2023;195:77-101. doi: 10.1007/978-1-0716-2918-5_5. Epub 2023 Feb 7.

DOI:10.1007/978-1-0716-2918-5_5
PMID:38585382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10997381/
Abstract

Adeno-associated viral vectors (AAV) are frequently used by neuroscientists to deliver tools, such as biosensors and optogenetic and chemogenetic actuators, . Despite its widespread use, AAV vector characterization and quality control can vary between labs and viral vector cores leading to variable results and irreproducibility. This protocol describes some of the characterization and quality control assays necessary to confirm an AAV vector's titer, genomic identity, serotype and purity.

摘要

腺相关病毒载体（AAV）被神经科学家广泛用于递送工具，如生物传感器、光遗传学和化学遗传学致动器。尽管其应用广泛，但不同实验室和病毒载体核心之间对AAV载体的表征和质量控制可能存在差异，从而导致结果可变且不可重复。本方案描述了一些用于确认AAV载体滴度、基因组同一性、血清型和纯度所需的表征和质量控制检测方法。

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本文引用的文献

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A Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts.
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