Jha Pankaj Kumar, Jaidumrong Tunyakamon, Rokaya Dinesh, Ovatlarnporn Chitchamai
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University Hat Yai Songkhla 90110 Thailand
Drug Delivery System Excellence Center, Prince of Songkla University Hat Yai Songkhla 90110 Thailand
RSC Adv. 2024 Apr 5;14(16):11017-11026. doi: 10.1039/d4ra01355g. eCollection 2024 Apr 3.
Antibiotics are of great interest due to antibiotic-resistant problems around the globe due to bacterial resistance to conventional antibiotics. In this study, a novel green biosynthesis of silver-ruthenium bimetallic zinc oxide nanocomposite using leaf extract as a reducing agent using zinc nitrate hexahydrate, silver nitrate, and ruthenium(iii) chloride as capping agents was reported. The results demonstrated that the surface morphology of the prepared bimetallic nanocomposite by scanning electron microscopy was hexagonal in shape for zinc nanoparticle, rectangular in shape for silver nanoparticle, and tetragonal in shape for ruthenium nanoparticle, having an average surface size 25, 35, and 55 nm, respectively. Fourier transform infrared analysis confirmed the presence of compounds containing alkene, halo-, sulfoxide, phenol, nitro-, phenyl-ester, carboxylic acid, amines, and alcohols which act as functional groups attached to the surface of nanocomposites. Results from X-ray diffraction analysis found 81.12% crystallinity and hexagonal structure of zinc nanoparticles, rectangular structure of silver nanoparticles, and tetragonal structure of ruthenium nanoparticles, which are also similar to the results from transmission electron microscopy analysis. The average size distribution by dynamic light scattering of silver-ruthenium bimetallic zinc oxide nanocomposite was 255 nm, which confirms the biosynthesis of non-uniform size. Photo-disinfection activity of a silver-ruthenium bimetallic zinc oxide nanocomposite against bacteria isolated from hospital wastewater under dark and ultraviolet-A irradiation conditions was observed. The antibacterial activity was calculated at 2.42704239, ensuring the silver-ruthenium bimetallic zinc oxide nanomaterials have photo-disinfection properties. The results from this study revealed that the developed novel antibacterial nanocomposite of silver-ruthenium bimetallic zinc oxide is useful in nanocoating photocatalytic disinfection and can be applied to disinfect surfaces.
由于全球范围内细菌对传统抗生素产生耐药性，导致抗生素耐药问题，抗生素备受关注。在本研究中，报道了一种新型绿色生物合成银-钌双金属氧化锌纳米复合材料的方法，该方法使用叶提取物作为还原剂，以六水合硝酸锌、硝酸银和氯化钌(iii)作为封端剂。结果表明，通过扫描电子显微镜观察，制备的双金属纳米复合材料的表面形态中，锌纳米颗粒为六边形，银纳米颗粒为矩形，钌纳米颗粒为四方晶形，平均表面尺寸分别为25、35和55 nm。傅里叶变换红外分析证实了含有烯烃、卤代、亚砜、酚、硝基、苯基酯、羧酸、胺和醇的化合物的存在，这些化合物作为连接在纳米复合材料表面的官能团。X射线衍射分析结果发现，锌纳米颗粒的结晶度为81.12%，具有六边形结构，银纳米颗粒为矩形结构，钌纳米颗粒为四方晶结构，这也与透射电子显微镜分析结果相似。银-钌双金属氧化锌纳米复合材料通过动态光散射测得的平均尺寸分布为255 nm，这证实了其非均匀尺寸的生物合成。观察了银-钌双金属氧化锌纳米复合材料在黑暗和紫外-A照射条件下对从医院废水中分离出的细菌的光消毒活性。计算得出抗菌活性为2.42704239，确保了银-钌双金属氧化锌纳米材料具有光消毒性能。本研究结果表明，所开发的新型银-钌双金属氧化锌抗菌纳米复合材料可用于纳米涂层光催化消毒，并可应用于表面消毒。
