Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), Granada, Spain.
Spanish National Research Council, Estación Experimental del Zaidín, Granada, Spain.
Methods Mol Biol. 2024;2798:1-9. doi: 10.1007/978-1-0716-3826-2_1.
Total antioxidant capacity (TAC) is a reliable indicator of antioxidant content in animal and plant samples. The different experimental approaches available allow the determination of TAC using, as a reference, diverse compounds with recognized antioxidant capacities such as Trolox, ascorbic acid, gallic acid, or melatonin. A new portable device, named BRS (BQC redox system), is now commercially available that, through an electrochemical approach, allows the determination of TAC in a simple, fast, reproducible, and robust way. In this chapter, using this portable device, a comparative analysis of the TAC is assayed in different red, citrus, and Solanaceae fruits, several Allium species, and organs of different plant species, including Arabidopsis thaliana. The obtained results demonstrate the versatility of the BRS portable device.
总抗氧化能力（TAC）是衡量动植物样本中抗氧化含量的可靠指标。现有的不同实验方法可通过使用具有公认抗氧化能力的不同化合物作为参考来确定 TAC，如 Trolox、抗坏血酸、没食子酸或褪黑素。一种名为 BRS（BQC 氧化还原系统）的新型便携式设备现已上市，它通过电化学方法以简单、快速、可重复和稳健的方式来确定 TAC。在本章中，使用该便携式设备对不同的红色、柑橘类和茄科水果、几种葱属物种以及包括拟南芥在内的不同植物物种的器官中的 TAC 进行了比较分析。所得结果证明了 BRS 便携式设备的多功能性。
