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锥虫中的钙调蛋白基因串联重复，并产生具有共同5'前导序列的多个mRNA。

Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.

作者信息

Tschudi C, Young A S, Ruben L, Patton C L, Richards F F

出版信息

Proc Natl Acad Sci U S A. 1985 Jun;82(12):3998-4002. doi: 10.1073/pnas.82.12.3998.

DOI:10.1073/pnas.82.12.3998
PMID:3858856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397921/
Abstract

In Trypanosoma brucei gambiense, the Ca2+ binding protein calmodulin is encoded by three identical tandemly repeated genes. The transcripts of these genes consist of several RNA species similar in size. A 35-nucleotide spliced leader sequence is present at the 5' end of each mRNA but is not encoded by DNA contiguous to these genes. We have identified two different sites for the fusion of the leader to the mRNA. These results strongly support the idea that a novel, possibly discontinuous, transcription mechanism is used by these parasites.

摘要

在布氏冈比亚锥虫中，钙离子结合蛋白钙调蛋白由三个相同的串联重复基因编码。这些基因的转录本由几种大小相似的RNA种类组成。每个mRNA的5'端都有一个35个核苷酸的剪接前导序列，但不是由与这些基因相邻的DNA编码的。我们已经确定了前导序列与mRNA融合的两个不同位点。这些结果有力地支持了这些寄生虫使用一种新的、可能不连续的转录机制的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c645/397921/7bbe21a7051c/pnas00352-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c645/397921/bad38ed94f0a/pnas00352-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c645/397921/7bbe21a7051c/pnas00352-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c645/397921/bad38ed94f0a/pnas00352-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c645/397921/7bbe21a7051c/pnas00352-0060-b.jpg

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1
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.锥虫中的钙调蛋白基因串联重复，并产生具有共同5'前导序列的多个mRNA。
Proc Natl Acad Sci U S A. 1985 Jun;82(12):3998-4002. doi: 10.1073/pnas.82.12.3998.
2
Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.多基因转录本是锥虫中钙调蛋白mRNA的前体。
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3
A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.在布氏锥虫中，一个5'剪接前导序列以反式方式添加到α-微管蛋白和β-微管蛋白转录本上。
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Role of Δ1-pyrroline-5-carboxylate dehydrogenase supports mitochondrial metabolism and host-cell invasion of Trypanosoma cruzi.Δ1-吡咯啉-5-羧酸脱氢酶的作用支持克氏锥虫的线粒体代谢和宿主细胞入侵。
J Biol Chem. 2015 Mar 20;290(12):7767-90. doi: 10.1074/jbc.M114.574525. Epub 2015 Jan 26.
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The calmodulin intergenic spacer as molecular target for characterization of Leishmania species.

本文引用的文献

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Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5' end.编码布氏锥虫可变表面糖蛋白的转录本在其5'端有一个短的、相同的外显子。
Gene. 1982 Dec;20(2):281-9. doi: 10.1016/0378-1119(82)90046-4.
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Cloning of cDNA Sequences Encoding the Calcium-Binding Protein, Calmodulin, from Barley (Hordeum vulgare L.).从大麦（Hordeum vulgare L.）中克隆编码钙结合蛋白钙调蛋白的cDNA序列。
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Cloning of a trypanosomatid gene coding for an ornithine decarboxylase that is metabolically unstable even though it lacks the C-terminal degradation domain.一种锥虫基因的克隆，该基因编码一种鸟氨酸脱羧酶，尽管它缺乏C末端降解结构域，但在代谢上不稳定。
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Evolution of EF-hand calcium-modulated proteins. IV. Exon shuffling did not determine the domain compositions of EF-hand proteins.EF 手型钙调蛋白的进化。IV. 外显子重排并未决定 EF 手型蛋白的结构域组成。
J Mol Evol. 1993 May;36(5):477-88. doi: 10.1007/BF02406723.
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Evolution of EF-hand calcium-modulated proteins. III. Exon sequences confirm most dendrograms based on protein sequences: calmodulin dendrograms show significant lack of parallelism.EF 手型钙调节蛋白的进化。III. 外显子序列证实了基于蛋白质序列的大多数系统树：钙调蛋白系统树显示出明显缺乏平行性。
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The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis.非肌肉型α-辅肌动蛋白中钙离子结合结构域的生化与遗传学分析
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Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei.布氏锥虫中两个不同葡萄糖转运蛋白基因家族的差异调控。
Mol Cell Biol. 1993 Feb;13(2):1146-54. doi: 10.1128/mcb.13.2.1146-1154.1993.
Cell. 1982 Jan;28(1):145-54. doi: 10.1016/0092-8674(82)90384-1.
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A cloned calmodulin structural gene probe is complementary to DNA sequences from diverse species.一个克隆的钙调蛋白结构基因探针与来自不同物种的DNA序列互补。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2330-4. doi: 10.1073/pnas.78.4.2330.
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Calmodulin.钙调蛋白
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Sequences homologous to variant antigen mRNA spliced leader in Trypanosomatidae which do not undergo antigenic variation.与锥虫科中不发生抗原变异的可变抗原mRNA剪接前导序列同源的序列。
Nature. 1984;308(5960):665-7. doi: 10.1038/308665a0.
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Proc Natl Acad Sci U S A. 1983 Nov;80(21):6485-9. doi: 10.1073/pnas.80.21.6485.
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