锥虫mRNA共享一个共同的5'剪接前导序列。
Trypanosome mRNAs share a common 5' spliced leader sequence.
作者信息
Parsons M, Nelson R G, Watkins K P, Agabian N
出版信息
Cell. 1984 Aug;38(1):309-16. doi: 10.1016/0092-8674(84)90552-x.
Abstract
A 5'-terminal leader sequence of 35 nucleotides was found to be present on multiple trypanosome RNAs. Based on its representation in cDNA libraries, we estimate that many, if not all, trypanosome mRNAs contain this leader. This same leader was originally identified on mRNAs encoding the molecules responsible for antigenic variation, variant surface glycoproteins. Studies of selected cDNAs containing this leader sequence revealed that leader-containing transcripts can be stage-specific, stage-regulated, or constitutive. They can be abundant or rare, and transcribed from single or multigene families. No linkage between the genomic leader sequences and the structural gene exons was observed. Possible mechanisms by which the leader sequences are added to trypanosome mRNAs are discussed.
摘要
在多种锥虫RNA上发现了一个35个核苷酸的5'端前导序列。根据其在cDNA文库中的表现，我们估计许多（如果不是全部的话）锥虫mRNA都含有这个前导序列。这个相同的前导序列最初是在编码负责抗原变异的分子——可变表面糖蛋白的mRNA上鉴定出来的。对含有该前导序列的选定cDNA的研究表明，含前导序列的转录本可以是阶段特异性的、阶段调控的或组成型的。它们可以丰富或稀少，并且可以从单基因家族或多基因家族转录而来。未观察到基因组前导序列与结构基因外显子之间的联系。文中讨论了前导序列添加到锥虫mRNA上的可能机制。
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