Hoogerbrugge P M, Wagemaker G, van Bekkum D W
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4268-9. doi: 10.1073/pnas.82.12.4268.
Hemopoietic stem cells as defined by the capacity to produce spleen colonies in lethally irradiated recipients were reported by P. F. Bartlett [(1982) Proc. Natl. Acad. Sci. USA 79, 2722-2725] to be present in high frequencies in mouse brain. He also reported similar numbers of colony-forming units, spleen (CFU-s), in the brains of Wf/Wf mice, the bone marrow of which lacks detectable spleen colony-forming cells. To verify these observations, single cell suspensions were produced from murine brains by incubation with trypsin and DNase, followed by removal of myelin by Percoll gradient centrifugation. Two to 13 CFU-s were detected per brain. This low number suggested contamination of the brains by either blood or bone marrow leaking from the skull bones during dissection. When the isolated, intact brains were washed carefully in balanced salt solution, the recovered number of CFU-s decreased to 0.1-0.4 per brain. No CFU-s could be detected in the brains of W/Wv mice. It is concluded that the CFU-s observed by Bartlett in preparations of mouse brain did not originate from the brain tissue.
P. F. 巴特利特[（1982年）《美国国家科学院院刊》79卷，2722 - 2725页]报道，在经致死剂量照射的受体小鼠中能够产生脾集落的造血干细胞在小鼠脑中以高频率存在。他还报道，在Wf/Wf小鼠的脑中存在数量相似的脾集落形成单位（CFU-s），而这些小鼠的骨髓中缺乏可检测到的脾集落形成细胞。为了验证这些观察结果，通过用胰蛋白酶和脱氧核糖核酸酶孵育从小鼠脑中制备单细胞悬液，然后通过Percoll梯度离心去除髓磷脂。每只脑检测到2至13个CFU-s。这个低数量表明在解剖过程中脑被来自颅骨的血液或骨髓污染。当将分离的完整脑在平衡盐溶液中仔细洗涤时，每只脑回收的CFU-s数量降至0.1 - 0.4个。在W/Wv小鼠的脑中未检测到CFU-s。结论是，巴特利特在小鼠脑制备物中观察到的CFU-s并非源自脑组织。
