Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Miyagi, Japan.
Monoclon Antib Immunodiagn Immunother. 2024 Apr;43(2):53-58. doi: 10.1089/mab.2024.0003.
The giant panda () is one of the important species in worldwide animal conservation. Because it is essential to understand the disease of giant panda for conservation, histopathological analyses of tissues are important to understand the pathogenesis. However, monoclonal antibodies (mAbs) against giant panda-derived proteins are limited. Podoplanin (PDPN) is an essential marker of lung type I alveolar epithelial cells, kidney podocytes, and lymphatic endothelial cells. PDPN is also overexpressed in various human tumors, which are associated with poor prognosis. Here, an anti-giant panda PDPN (gpPDPN) mAb, PMab-314 (mouse IgG, kappa) was established using the Cell-Based Immunization and Screening method. PMab-314 recognized N-terminal PA16-tagged gpPDPN-overexpressed Chinese hamster ovary-K1 cells (CHO/PA16-gpPDPN) in flow cytometry. The value of PMab-314 for CHO/PA16-gpPDPN was determined as 1.3 × 10 M. Furthermore, PMab-314 is useful for detecting gpPDPN in western blot analysis. These findings indicate that PMab-314 is a useful tool for the analyses of gpPDPN-expressed cells.
大熊猫（）是全球动物保护中重要的物种之一。由于了解大熊猫的疾病对于保护工作至关重要，因此对组织进行组织病理学分析对于了解发病机制非常重要。然而，针对大熊猫来源的蛋白质的单克隆抗体（mAb）却非常有限。足突蛋白（PDPN）是肺 I 型肺泡上皮细胞、肾脏足细胞和淋巴管内皮细胞的重要标志物。PDPN 在各种人类肿瘤中也过表达，与预后不良相关。在这里，使用基于细胞的免疫接种和筛选方法建立了针对大熊猫 PDPN（gpPDPN）的单克隆抗体（mAb）PMab-314（小鼠 IgG，kappa）。PMab-314 在流式细胞术中识别表达 N 端 PA16 标签的 gpPDPN 过表达中国仓鼠卵巢-K1 细胞（CHO/PA16-gpPDPN）。PMab-314 对 CHO/PA16-gpPDPN 的 值确定为 1.3×10 M。此外，PMab-314 可用于 Western blot 分析中检测 gpPDPN。这些发现表明，PMab-314 是分析表达 gpPDPN 的细胞的有用工具。
