Chaikritsadakarn Amornrat, Witthayawirasak Banchong, Muenhor Dudsadee, DeLaune Ronald D, Muenpo Chutchawan
Faculty of Environmental Management, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Center of Excellence on Hazardous Substance Management (HSM), Bangkok, 10330, Thailand.
Heliyon. 2024 Mar 26;10(7):e28377. doi: 10.1016/j.heliyon.2024.e28377. eCollection 2024 Apr 15.
Di (2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in plastics. The effects of DEHP on terrestrial vertebrates have been extensively reported but the effects of DEHP contamination on aquatic ecosystems have not been thoroughly studied. Since water bodies are one of the main mediums through which DEHP is released worldwide, the impacts of DEHP contamination should be manifested in water fleas. Therefore, maternal were exposed to 1, 10, 100, and 1000 μg/L concentrations of DEHP. Changes in growth and reproduction were evaluated. The findings demonstrated that DEHP exposure did not have a negative impact on growth or the ability to reproduce. An analysis of the ovary yolk body (YB) demonstrated that the average size and number of yolk bodies (YBs) produced by exposed to 1000 μg/L DEHP were not significantly different to the average size and number of YBs produced in blank control and solvent control conditions. These outcomes support the cellular pathology data gathered by other researchers. Nevertheless, when was exposed to 1000 μg/L DEHP for five days, a significant increase in YB numbers was observed with changes in YB morphology. The critical cellular pathology of YB showed morphological abnormalities, including rod-shaped YBs, and YB density was higher than in the blank and solvent controls. Even though these results suggest that antioxidative stress can be induced by DEHP exposure, growth, and reproduction were not significantly different among exposed water fleas compared to fleas in the blank and solvent controls. The result was attributed to the antioxidant response of the water flea. In conclusion, the present study enhances our understanding of previous findings from risk assessments of DEHP contamination in aquatic ecosystems.
邻苯二甲酸二（2-乙基己基）酯（DEHP）在塑料中用作增塑剂。DEHP对陆生脊椎动物的影响已有大量报道，但DEHP污染对水生生态系统的影响尚未得到充分研究。由于水体是全球范围内DEHP释放的主要介质之一，DEHP污染的影响应在水蚤中体现。因此，将母体暴露于浓度为1、10、100和1000μg/L的DEHP中。评估生长和繁殖的变化。研究结果表明，暴露于DEHP对生长或繁殖能力没有负面影响。对卵巢卵黄体（YB）的分析表明，暴露于1000μg/L DEHP的母体产生的卵黄体（YB）的平均大小和数量与空白对照和溶剂对照条件下产生的YB的平均大小和数量没有显著差异。这些结果支持了其他研究人员收集的细胞病理学数据。然而，当母体暴露于1000μg/L DEHP五天时，观察到YB数量显著增加，且YB形态发生变化。YB的关键细胞病理学表现为形态异常，包括杆状YB，且YB密度高于空白对照和溶剂对照。尽管这些结果表明DEHP暴露可诱导抗氧化应激，但与空白对照和溶剂对照中的水蚤相比，暴露于DEHP的水蚤在生长和繁殖方面没有显著差异。该结果归因于水蚤的抗氧化反应。总之，本研究增进了我们对先前水生生态系统中DEHP污染风险评估结果的理解。
