Jiang Dandan, Zhou Zheng, Shen Yufeng, Tang Xiaoxue, Gou Xiaorui, Huang Meiyu, Tong Yizhou, Chen Miaomiao, Yu Chong-Qing
Dept. of Stomatology, the First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832000, China.
Dept. of Stomatology, Shihezi University, Shihezi 832000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Jan 25;40(1):45-51. doi: 10.7518/hxkq.2022.01.007.
This study aims to detect the levels of mucin (MUC)-4, metalloproteinase (MMP)-7, and MMP-8 in peri-implant crevicular fluid (PICF) and investigate whether the novel combinations of MMP-7 and MUC-4 are effective markers of peri-implant diseases, particularly when used in the PICF of healthy individuals, to provide a theoretical basis for finding a novel reference index that can aid the diagnosis, evaluation, and treatment of peri-implant diseases.
A total of 63 subjects with 2-5 years of upper prosthesis loading were selected according to inclusion and exclusion criteria, composed of 24 controls and 39 patients with peri-implantitis (PI) group. MUC-4, MMP-7, and MMP-8 levels were detected through enzyme linked immunosorbent assay (ELISA).
No significant differences in age, sex, and other parameters were found between the PI and control groups. The PI group had higher MMP-7 and MMP-8 expression levels (<0.05) but lower MUC-4 level (<0.001). Correlation analysis showed that MMP-7 was positively correlated with pocket probing depth (PPD) (=0.451, <0.001); MMP-8 was positively correlated with PPD, bleeding on probing (BOP), and gingival index (GI) (=0.619, <0.001; =0.478, <0.001; =0.332, =0.009). MUC-4 was negatively correlated with PPD, BOP, and GI (=-0.492, <0.001; =-0.321, =0.010; =-0.396, =0.001). MMP-7, MMP-8, and MUC-4 had certain diagnostic efficacy for PI. MMP-8 exhibited the best diagnostic efficacy for PI. When the cutoff value of MMP-8 was >21.21, the area under the curve (AUC) was 0.868, and the sensitivity and specificity for the diagnosis of PI were 0.96 and 0.68, respectively. The diagnostic efficacy of MMP-7 and MUC-4 parallel diagnostic models was higher than that of each factor, and the diagnostic sensitivity of the model for PI was 0.96, and the specificity was 0.56.
Differences in MMP-7 and MUC-4 levels were found between the inflammation and control groups and may be diagnostic indicators for predicting PI; combinations of MMP-7 and MUC-4 had a good diagnostic value for inflammation.
本研究旨在检测种植体周围龈沟液（PICF）中黏蛋白（MUC）-4、金属蛋白酶（MMP）-7和MMP-8的水平，并研究MMP-7与MUC-4的新组合是否为种植体周围疾病的有效标志物，尤其是在健康个体的PICF中使用时，为寻找有助于种植体周围疾病诊断、评估和治疗的新参考指标提供理论依据。
根据纳入和排除标准，共选取63例佩戴上部修复体2 - 5年的受试者，包括24例对照组和39例种植体周围炎（PI）患者组。通过酶联免疫吸附测定（ELISA）检测MUC-4、MMP-7和MMP-8水平。
PI组和对照组在年龄、性别及其他参数方面无显著差异。PI组MMP-7和MMP-8表达水平较高（<0.05），但MUC-4水平较低（<0.001）。相关性分析显示，MMP-7与牙周袋探诊深度（PPD）呈正相关（=0.451，<0.001）；MMP-8与PPD（=0.619，<0.001）、探诊出血（BOP）（=0.478，<0.001）和牙龈指数（GI）（=0.332，=0.009）呈正相关。MUC-4与PPD、BOP和GI呈负相关（=-0.492，<0.001；=-0.321，=0.010；=-0.396，=0.001）。MMP-7、MMP-8和MUC-4对PI有一定的诊断效能。MMP-8对PI的诊断效能最佳。当MMP-8的截断值>21.21时，曲线下面积（AUC）为0.868，诊断PI的敏感性和特异性分别为0.96和
