Wahlström T, Koskimies A I, Tenhunen A, Rutanen E M, Yki-Järvinen H, Julkunen M, Sjöberg J, Seppälä M
Ann N Y Acad Sci. 1985;442:402-7. doi: 10.1111/j.1749-6632.1985.tb37546.x.
Placental proteins PP10, PP12, and PP14 and pregnancy-associated plasma protein A (PAPP-A) and relaxin (RX) were studied by the immunoperoxidase method in the uterine mucosa at the time of embryo replacement in 18 women for whom no embryo was available to be replaced. All subjects had received 17 alpha-hydroxyprogesterone caproate at the time of follicle aspiration, which had been performed 36 hours after preovulatory administration of hCG. The time between follicle aspiration and endometrial biopsy varied from 26 to 216 hours. PP10 was not detected in any specimen. RX was found in 17 of 18, PP12 in 15 of 18, PAPP-A in 14 of 18, and PP14 in 6 of 18 specimens. In a normal cycle, PP12, PAPP-A and RX appear in the endometrium on the fourth day post ovulation. In the conditions described above the same proteins could be seen in the endometrium sooner after oocyte retrieval. This may be due to a strong progesterone effect after multiple follicle aspirations and treatment with 17 alpha-hydroxyprogesterone. The significance of the occurrence of these pregnancy proteins as well as that of the morphologic and biochemical maturity of the endometrium at the time of embryo transfer are discussed.
采用免疫过氧化物酶法，对18例无可移植胚胎的妇女在胚胎移植时的子宫黏膜中的胎盘蛋白PP10、PP12和PP14、妊娠相关血浆蛋白A（PAPP - A）及松弛素（RX）进行了研究。所有受试者在卵泡抽吸时均接受了己酸17α - 羟孕酮，卵泡抽吸在排卵前注射hCG后36小时进行。卵泡抽吸与子宫内膜活检之间的时间间隔为26至216小时。在任何标本中均未检测到PP10。18例中有17例检测到RX，18例中有15例检测到PP12，18例中有14例检测到PAPP - A，18例中有6例检测到PP14。在正常周期中，PP12、PAPP - A和RX在排卵后第4天出现在子宫内膜中。在上述情况下，卵母细胞取出后不久，在子宫内膜中就能见到相同的蛋白质。这可能是由于多次卵泡抽吸及使用己酸17α - 羟孕酮治疗后产生了强烈的孕酮作用。文中讨论了这些妊娠蛋白出现的意义以及胚胎移植时子宫内膜形态学和生化成熟度的意义。
