Dong Xiaoyan, Tu Chen, Xie Zhihong, Luo Yongming, Zhang Lei, Li Zhaoyi
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment of Shandong Agricultural University, Taian 271000, China.
Microorganisms. 2022 Apr 1;10(4):767. doi: 10.3390/microorganisms10040767.
Root colonization and plant probiotic function are important traits of plant growth-promoting rhizobacteria (PGPR). SC60, a plant endophytic strain screened from has a strong colonization ability on various plant roots, which indicates that SC60 has a preferable adaptability to plants. However, the probiotic function of the strain SC60 is not well-understood. Promoting plant growth and suppressing soil-borne pathogens are key to the plant probiotic functions. In this study, the genetic mechanism of plant growth-promoting and antibacterial activity of the strain SC60 was analyzed by biological and bioinformatics methods. The complete genome size of strain SC60 was 3,962,671 bp, with 4079 predicted genes and an average GC content of 46.46%. SC60 was designated as according to the comparative analysis, including average nucleotide polymorphism (ANI), digital DNA-DNA hybridization (dDDH), and phylogenetic analysis. Genomic secondary metabolite analyses indicated two clusters encoding potential new antimicrobials. The antagonism experiments revealed that strain SC60 had the ability to inhibit the growth of a variety of plant pathogens and its closely related strains of spp., which was crucial to the rhizospheric competitiveness and growth-promoting effect of the strain. The present results further suggest that SC60 could be used as a PGPR strain to develop new biocontrol agents or microbial fertilizers.
根系定殖和植物益生菌功能是植物促生根际细菌（PGPR）的重要特性。从[具体来源未给出]筛选出的植物内生菌株SC60对多种植物根系具有很强的定殖能力，这表明SC60对植物具有较好的适应性。然而，菌株SC60的益生菌功能尚未得到充分了解。促进植物生长和抑制土传病原菌是植物益生菌功能的关键。在本研究中，通过生物学和生物信息学方法分析了菌株SC60促进植物生长和抗菌活性的遗传机制。菌株SC60的完整基因组大小为3,962,671 bp，预测基因有4079个，平均GC含量为46.46%。根据平均核苷酸多态性（ANI）、数字DNA-DNA杂交（dDDH）和系统发育分析等比较分析，SC60被归类为[具体类别未给出]。基因组次生代谢物分析表明有两个簇编码潜在的新型抗菌物质。拮抗实验表明，菌株SC60具有抑制多种植物病原菌及其密切相关的[具体菌种未给出]菌株生长的能力，这对该菌株的根际竞争力和促生长效果至关重要。目前的结果进一步表明，SC60可作为一种PGPR菌株用于开发新型生物防治剂或微生物肥料。
