Department of Orthopedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.
Department of Sports Medicine, Lianyungang Clinical College of Nanjing Medical University, Lianyungang, Jiangsu, People's Republic of China.
Int J Nanomedicine. 2024 Apr 6;19:3275-3293. doi: 10.2147/IJN.S449952. eCollection 2024.
This study aims to explore a novel scaffold for osteotendinous junction regeneration and to preliminarily verify its osteogenic and tenogenic abilities in vitro.
A polycaprolactone (PCL) scaffold with aligned and orthogonal fibers was created using melt electrowriting (MEW) and fused deposition modeling (FDM). The scaffold was coated with Type I collagen, and hydroxyapatite was carefully added to separate the regions intended for bone and tendon regeneration, before being rolled into a cylindrical shape. Human adipose-derived stem cells (hADSCs) were seeded to evaluate viability and differentiation. Scaffold characterization was performed with Scanning Electron Microscope (SEM). Osteogenesis was assessed by alkaline phosphatase (ALP) and Alizarin red staining, while immunostaining and transcription-quantitative polymerase chain reaction (RT-qPCR) evaluated osteogenic and tendogenic markers.
Scaffolds were developed in four variations: aligned (A), collagen-coated aligned (A+C), orthogonal (O), and mineral-coated orthogonal (O+M). SEM analysis confirmed surface morphology and energy-dispersive X-ray spectroscopy (EDS) verified mineral coating on O+M types. Hydrophilicity and mechanical properties were optimized in modified scaffolds, with A+C showing increased tensile strength and O+M improved in compression. hADSCs demonstrated good viability and morphology across scaffolds, withO+M scaffolds showing higher cell proliferation and osteogenic potential, and A and A+C scaffolds supporting tenogenic differentiation.
This study confirms the potential of a novel PCL scaffold with distinct regions for osteogenic and tenogenic differentiation, supporting the regeneration of osteotendinous junctions in vitro.
本研究旨在探索一种新型的用于骨腱结合部再生的支架，并初步验证其在体外的成骨和成腱能力。
采用熔融静电纺丝（MEW）和熔丝制造（FDM）技术制备了具有定向和正交纤维的聚己内酯（PCL）支架。支架用 I 型胶原包被，并小心地添加羟基磷灰石，将其分为用于骨和腱再生的区域，然后将其卷成圆柱形。人脂肪来源干细胞（hADSCs）被接种以评估其活力和分化。采用扫描电子显微镜（SEM）对支架进行特征描述。通过碱性磷酸酶（ALP）和茜素红染色评估成骨作用，免疫染色和转录定量聚合酶链反应（RT-qPCR）评估成骨和成腱标记物。
共开发了四种支架：定向（A）、胶原包被定向（A+C）、正交（O）和矿化正交（O+M）。SEM 分析证实了表面形态，能谱分析（EDS）证实了 O+M 型的矿化涂层。改良支架优化了亲水性和机械性能，其中 A+C 型支架的拉伸强度增加，O+M 型的压缩强度提高。hADSCs 在各种支架上均表现出良好的活力和形态，其中 O+M 支架的细胞增殖和成骨潜能更高，A 和 A+C 支架支持腱分化。
本研究证实了一种具有独特成骨和成腱分化区域的新型 PCL 支架的潜力，支持了体外骨腱结合部的再生。
