Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sci Rep. 2022 Jul 20;12(1):12359. doi: 10.1038/s41598-022-15602-y.
Bone tissue engineering uses various methods and materials to find suitable scaffolds that regenerate lost bone due to disease or injury. Poly(ε-caprolactone) (PCL) can be used in 3D printing for producing biodegradable scaffolds by fused deposition modeling (FDM). However, the hydrophobic surfaces of PCL and its non-osteogenic nature reduces adhesion and cell bioactivity at the time of implantation. This work aims to enhance bone formation, osteogenic differentiation, and in vitro biocompatibility via PCL scaffolds modification with Hydroxyapatite (HA) and Collagen type I (COL). This study evaluated the osteosupportive capacity, biological behavior, and physicochemical properties of 3D-printed PCL, PCL/HA, PCL/COL, and PCL/HA/COL scaffolds. Biocompatibility and cells proliferation were investigated by seeding human adipose tissue-derived mesenchymal stem cells (hADSCs) onto the scaffolds, which were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and 6-diamidino-2-phenylindole (DAPI) staining. In addition, the bone differentiation potential of the hADSCs was assessed using calcium deposition, alkaline phosphatase (ALP) activity, and bone-related protein and genes. Although all constructed scaffolds support hADSCs proliferation and differentiation, the results showed that scaffold coating with HA and COL can boost these capacities in a synergistic manner. According to the findings, the tricomponent 3D-printed scaffold can be considered as a promising choice for bone tissue regeneration and rebuilding.
骨组织工程利用各种方法和材料来寻找合适的支架，以再生因疾病或损伤而丢失的骨。聚己内酯（PCL）可用于 3D 打印，通过熔融沉积建模（FDM）生产可生物降解的支架。然而，PCL 的疏水面及其非成骨性会降低植入时的细胞黏附和生物活性。本工作旨在通过在 PCL 支架上修饰羟基磷灰石（HA）和 I 型胶原（COL）来增强骨形成、成骨分化和体外生物相容性。本研究评估了 3D 打印的 PCL、PCL/HA、PCL/COL 和 PCL/HA/COL 支架的骨支持能力、生物学行为和物理化学性能。通过将人脂肪组织来源的间充质干细胞（hADSCs）接种到支架上，研究了生物相容性和细胞增殖情况，通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测和 6-二脒基-2-苯基吲哚（DAPI）染色分析细胞增殖情况。此外，通过钙沉积、碱性磷酸酶（ALP）活性以及与骨相关的蛋白和基因评估了 hADSCs 的成骨分化潜力。虽然所有构建的支架都支持 hADSCs 的增殖和分化，但结果表明，支架表面涂覆 HA 和 COL 可以以协同方式增强这些能力。根据研究结果，这种三组分 3D 打印支架可被视为骨组织再生和重建的有前途的选择。
