Jiang Xiping, Wu Shaohua, Kuss Mitchell, Kong Yunfan, Shi Wen, Streubel Philipp N, Li Tieshi, Duan Bin
Mary & Dick Holland Regenerative Medicine Program, Division of Cardiology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Molecular Genetics and Cell Biology Program, Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Bioact Mater. 2020 May 7;5(3):636-643. doi: 10.1016/j.bioactmat.2020.04.017. eCollection 2020 Sep.
Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties, as well as controllable microenvironments for tendon regeneration. In this study, we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic--glycolic acid (PLGA) with cell-laden collagen-fibrin hydrogels. We designed and fabricated two types of scaffolds: one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole. Uniaxial tensile tests showed that both types of scaffolds had improved mechanical properties compared to single-layered PLGA scaffolds. The printed scaffold with collagen-fibrin hydrogels effectively supported the growth, proliferation, and tenogenic differentiation of human adipose-derived mesenchymal stem cells. Subcutaneous implantation of the multilayered scaffolds demonstrated their excellent biocompatibility. This study demonstrates the feasibility of 3D printing multilayered scaffolds for application in rotator cuff tendon regeneration.
由于手术干预后再撕裂率高，修复巨大的肩袖肌腱缺损仍然是一项挑战。3D打印已成为一种有前景的技术，能够制造具有异质结构和机械性能的工程组织，以及用于肌腱再生的可控微环境。在本研究中，我们通过将聚乳酸-乙醇酸（PLGA）的3D打印支架与负载细胞的胶原-纤维蛋白水凝胶相结合，开发了一种肩袖肌腱修复的新策略。我们设计并制造了两种类型的支架：一种具有单独的逐层结构，另一种整体为三层结构。单轴拉伸试验表明，与单层PLGA支架相比，这两种类型的支架都具有改善的机械性能。带有胶原-纤维蛋白水凝胶的打印支架有效地支持了人脂肪来源间充质干细胞的生长、增殖和腱分化。多层支架的皮下植入证明了它们优异的生物相容性。本研究证明了3D打印多层支架应用于肩袖肌腱再生的可行性。
