Post-Graduate Specialization School in Food Inspection "G. Tiecco", Department of Veterinary Medicine, University of Teramo, Strada Provinciale 18, 64100, Piano d'Accio, Teramo, Italy.
SIAOA Veterinary Public Service, Ascoli Piceno, 63100, Italy.
J Appl Microbiol. 2024 Apr 1;135(4). doi: 10.1093/jambio/lxae098.
This study investigated phenotypic and genotypic antimicrobial resistance profiles of Vibrio strains identified from Mytilus galloprovincialis farmed for human consumption in the Adriatic Sea Central Italy.
A total of 475 mussels (M. galloprovincialis) were involved in the present study, and culture-dependent microbiological methods permitted to identify a total of 50 Vibrio strains that were tested for antibiotic susceptibility followed by the genetic determinant detections. Antibiograms showed resistance against ampicillin (36.0%), amoxicillin-clavulanic acid (30.0%), gentamycin (14.0%), and imipenem (18.0%). Biomolecular assays amplified a total of 264 antibiotic resistance genes harbored by both susceptible and resistant Vibrio species. Among resistance genes, aacC2 (62.0%) and aadA (58.0%) for aminoglycosides, blaTEM (54.0%) for beta-lactams, qnrS (24.0%) for quinolones, tetD (66.0%) for tetracyclines, and vanB (60.0%) for glycopeptides were mainly amplified by PCR assays.
Vibrio genus is involved in the antibiotic resistance phenomenon diffusion in the aquatic environments, as demonstrated by the harboring of many genetic determinants representing a kind of genetic "dark world".
本研究调查了来自意大利亚得里亚海中部养殖贻贝（Mytilus galloprovincialis）中的 50 株弧菌的表型和基因型抗微生物耐药谱。
本研究共涉及 475 只贻贝（M. galloprovincialis），通过依赖培养的微生物学方法共鉴定出 50 株弧菌，并对其进行了抗生素药敏试验，随后检测了遗传决定簇。药敏试验结果显示，弧菌对氨苄西林（36.0%）、阿莫西林-克拉维酸（30.0%）、庆大霉素（14.0%）和亚胺培南（18.0%）耐药。生物分子检测共扩增了 264 种抗生素耐药基因，这些基因存在于敏感和耐药的弧菌物种中。在耐药基因中，aacC2（62.0%）和 aadA（58.0%）用于氨基糖苷类，blaTEM（54.0%）用于β-内酰胺类，qnrS（24.0%）用于喹诺酮类，tetD（66.0%）用于四环素类，vanB（60.0%）用于糖肽类，这些基因主要通过 PCR 检测扩增。
弧菌属参与了水生环境中的抗生素耐药现象的扩散，这是由许多遗传决定簇所证明的，这些遗传决定簇代表了一种遗传“黑暗世界”。
