Zhang Hang, Yang Sheng, Chen Man, Tian Dai-Shi, Qin Chuan
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology;
J Vis Exp. 2022 Apr 5(182). doi: 10.3791/63511.
Microglia, the resident innate immune cells in the brain, are the primary responders to inflammation or injury in the central nervous system (CNS). Microglia can be divided into resting state and activated state and can rapidly change state in response to the microenvironment of the brain. Microglia will be activated under different pathological conditions and exhibit different phenotypes. In addition, there are many different subgroups of activated microglia and great heterogeneity between different subgroups. The heterogeneity mainly depends on the molecular specificity of microglia. Studies have revealed that microglia will be activated and play an important role in the pathological process of inflammatory demyelination. To better understand the characteristics of microglia in inflammatory demyelinating diseases such as multiple sclerosis and neuromyelitis optica spectrum disorder, we propose a perilesional primary microglial sorting protocol. This protocol utilizes columnar magnetic-activated cell sorting (MACS) to obtain highly purified primary microglia and preserve the molecular characteristics of microglia to investigate the potential effects of microglia in inflammatory demyelinating diseases.
小胶质细胞是大脑中的常驻固有免疫细胞，是中枢神经系统（CNS）炎症或损伤的主要应答者。小胶质细胞可分为静息状态和激活状态，并能根据大脑的微环境迅速改变状态。在不同的病理条件下，小胶质细胞会被激活并表现出不同的表型。此外，激活的小胶质细胞有许多不同的亚群，不同亚群之间存在很大的异质性。这种异质性主要取决于小胶质细胞的分子特异性。研究表明，小胶质细胞在炎性脱髓鞘的病理过程中会被激活并发挥重要作用。为了更好地了解小胶质细胞在多发性硬化症和视神经脊髓炎谱系障碍等炎性脱髓鞘疾病中的特征，我们提出了一种病损周围原发性小胶质细胞分选方案。该方案利用柱状磁珠激活细胞分选（MACS）来获得高度纯化的原发性小胶质细胞，并保留小胶质细胞的分子特征，以研究小胶质细胞在炎性脱髓鞘疾病中的潜在作用。
