Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Research Center of Super Rice Engineering and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
School of Life Sciences, Jinggangshan University, Ji'an, 343009, China.
BMC Ecol Evol. 2021 Jul 9;21(1):141. doi: 10.1186/s12862-021-01873-y.
Multidrug and toxic compound extrusion (MATE) proteins are involved in many physiological functions of plant growth and development. Although an increasing number of MATE proteins have been identified, the understanding of MATE proteins is still very limited in rice. In this study, 46 MATE proteins were identified from the rice (Oryza sativa) genome by homology searches and domain prediction. The rice MATE family was divided into four subfamilies based on the phylogenetic tree. Tandem repeats and fragment replication contribute to the expansion of the rice MATE gene family. Gene structure and cis-regulatory elements reveal the potential functions of MATE genes. Analysis of gene expression showed that most of MATE genes were constitutively expressed and the expression patterns of genes in different tissues were analyzed using RNA-seq. Furthermore, qRT-PCR-based analysis showed differential expression patterns in response to salt and drought stress. The analysis results of this study provide comprehensive information on the MATE gene family in rice and will aid in understanding the functional divergence of MATE genes.
多药和毒物外排（MATE）蛋白参与植物生长和发育的许多生理功能。尽管已经鉴定出越来越多的 MATE 蛋白，但在水稻中对 MATE 蛋白的了解仍然非常有限。在这项研究中，通过同源搜索和结构域预测，从水稻（Oryza sativa）基因组中鉴定出 46 种 MATE 蛋白。根据系统发育树，水稻 MATE 家族分为四个亚家族。串联重复和片段复制有助于扩展水稻 MATE 基因家族。基因结构和顺式调控元件揭示了 MATE 基因的潜在功能。基因表达分析表明，大多数 MATE 基因是组成型表达的，并且使用 RNA-seq 分析了不同组织中基因的表达模式。此外，基于 qRT-PCR 的分析表明，它们对盐和干旱胁迫的反应存在差异表达模式。这项研究的分析结果为水稻 MATE 基因家族提供了全面的信息，并将有助于理解 MATE 基因的功能分化。
