State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Int J Mol Sci. 2024 Jun 24;25(13):6898. doi: 10.3390/ijms25136898.
, a plant-specific gene, is known for its involvement in splicing group II introns within maize chloroplast. However, its role in chloroplast development and global gene expression remains poorly understood. This study aimed to investigate the role of in chloroplast development and identify the genes that mediate its function in the development of entire tomato plants. Consistent with findings in maize, silencing induced dwarfism and leaf whitening in tomato plants. Subcellular localization analysis revealed that the RNC1 protein is localized to both the nucleus and cytoplasm, including the stress granule and chloroplasts. Electron microscopic examination of tomato leaf transverse sections exposed significant disruptions in the spatial arrangement of the thylakoid network upon silencing, crucial for efficient light energy capture and conversion into chemical energy. Transcriptome analysis suggested that silencing potentially impacts tomato plant development through genes associated with all three categories (biological processes, cellular components, and molecular functions). Overall, our findings contribute to a better understanding of the critical role of in chloroplast development and its significance in plant physiology.
, 一种植物特异性基因，已知其参与玉米叶绿体中 II 组内含子的剪接。然而，其在叶绿体发育和全局基因表达中的作用仍知之甚少。本研究旨在探究 在叶绿体发育中的作用，并确定介导其在整个番茄植物发育中功能的基因。与在玉米中的发现一致， 沉默诱导番茄植株矮化和叶片白化。亚细胞定位分析表明，RNC1 蛋白定位于细胞核和细胞质，包括应激颗粒和叶绿体。对番茄叶片横切片的电子显微镜检查显示，在 沉默后，类囊体网络的空间排列发生了显著的破坏，这对于有效捕获光能并将其转化为化学能至关重要。转录组分析表明， 沉默可能通过与三个类别（生物过程、细胞成分和分子功能）相关的基因对番茄植物发育产生影响。总的来说，我们的研究结果有助于更好地理解 在叶绿体发育中的关键作用及其在植物生理学中的重要性。
