Key Laboratory for Molecular Medicine and Chinese Medicine Preparations, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310002, China; College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China.
Key Laboratory for Molecular Medicine and Chinese Medicine Preparations, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310002, China; College of Pharmacy Science, Zhejiang University of Technology, Hangzhou 310027, China.
Fitoterapia. 2024 Jun;175:105959. doi: 10.1016/j.fitote.2024.105959. Epub 2024 Apr 12.
Lysimachia capillipes Hemsl., a traditional Chinese medicine (TCM), is commonly prescribed for its anti-inflammatory and anti-tumor properties. Pharmacological studies have demonstrated that Lysimachia capillipes Hemsl. saponins (LCS) are the primary bioactive component. However, its mechanism for treating colorectal cancer (CRC) is still unknown. Increasing evidence suggests a close relationship between CRC, intestinal flora, and host metabolism. Thus, this study aims to investigate the mechanism of LCS amelioration of CRC from the perspective of the gut microbiome and metabolome. As a result, seven gut microbiotas and fourteen plasma metabolites were significantly altered between the control and model groups. Among them, one gut microbiota genera (Monoglobus) and six metabolites (Ureidopropionic acid, Cytosine, L-Proline, 3-hydroxyanthranilic acid, Cyclic AMP and Suberic acid) showed the most pronounced callback trend after LCS administration. Subsequently, the correlation analysis revealed significant associations between 68 pairs of associated metabolites and gut microbes, with 13 pairs of strongly associated metabolites regulated by the LCS. Taken together, these findings indicate that the amelioration of CRC by LCS is connected to the regulation of intestinal flora and the recasting of metabolic abnormalities. These insights highlight the potential of LCS as a candidate drug for the treatment of CRC.
金钱草（Lysimachia capillipes Hemsl.）是一种传统的中药，因其具有抗炎和抗肿瘤的特性而被广泛应用。药理学研究表明，金钱草皂苷（LCS）是其主要的生物活性成分。然而，其治疗结直肠癌（CRC）的机制尚不清楚。越来越多的证据表明 CRC 与肠道菌群和宿主代谢之间存在密切关系。因此，本研究旨在从肠道微生物组和代谢组学的角度探讨 LCS 改善 CRC 的作用机制。结果显示，对照组和模型组之间有 7 种肠道微生物群和 14 种血浆代谢物发生了显著变化。其中，一个肠道微生物属（Monoglobus）和 6 种代谢物（尿嘧啶丙酸、胞嘧啶、L-脯氨酸、3-羟基邻氨基苯甲酸、环磷酸腺苷和琥珀酸）在 LCS 给药后回调趋势最为明显。随后，相关性分析显示，相关代谢物和肠道微生物之间存在 68 对显著关联，其中 13 对受 LCS 强烈调节的关联代谢物。综上所述，这些发现表明 LCS 通过调节肠道菌群和重塑代谢异常来改善 CRC。这些研究结果突出了 LCS 作为 CRC 治疗候选药物的潜力。
