College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China.
Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical of College, Hangzhou 310014, China.
J Chromatogr Sci. 2021 Oct 29;59(10):941-948. doi: 10.1093/chromsci/bmab028.
Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.
丁香苷 B（CPS-B）和丁香苷 C（CPS-C）作为金钱草中的关键成分，由于其良好的生物活性，越来越引起许多研究人员的兴趣和研究关注。如今，CPS-B 和 CPS-C 的参考标准产量非常有限。由于参考标准的缺乏，测定可能难以进行，随后的质量控制和评价也将受到限制。为了解决这个紧迫的问题，提出并建立了基于高效液相色谱-串联蒸发光散射检测器的一种单标记多成分定量分析（QAMS）方法。在这种 QAMS 方法中，通过易于获得的芦丁苷 IV 作为外标，可以计算出两种生物活性成分的含量。并对该方法的方法学实验进行了评价，表明了该 QAMS 方法的准确性、稳定性和可行性。因此，在本研究中，该建立的方法将适当地满足金钱草中 CPS-B、CPS-C 的测定和质量控制要求。
