Ju Yikun, Zhu Fang, Fang Bairong
Department of Plastic and Aesthetic (Burn) Surgery, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Int J Med Sci. 2024 Mar 31;21(5):921-936. doi: 10.7150/ijms.93044. eCollection 2024.
Although is dysregulated in several tumors, its role in head and neck squamous cell carcinoma (HNSC) is not fully understood. The aim of this study was to analyze the role of in HNSC. The clinical information and expression data of HNSC were mined from the TCGA/GEO/cbioportal database. The correlation between expression and immune cell infiltration in HNSC tumors was analyzed by bioinformatics and gene enrichment analysis was performed. was silenced in HNSC cell lines, and changes at the genetic and molecular levels were verified through qRT-PCR and Western blotting. The researchers also validated its functional phenotype through a series of cell function experiments. The results showed that overexpression and copy number variation of in HNSC were associated with poorer prognosis. In addition, expression was significantly negatively correlated with immune cell infiltration. Silencing of in HNSC cells significantly reduced the rate of cell migration. may affect the progression of HNSC by regulating epithelial-mesenchymal transition. In conclusion, is a potential biomarker of HNSC prognosis and a potential target for immunotherapy.
尽管[具体基因名称未给出]在多种肿瘤中表达失调，但其在头颈部鳞状细胞癌（HNSC）中的作用尚未完全明确。本研究旨在分析[具体基因名称未给出]在HNSC中的作用。从TCGA/GEO/cbioportal数据库中挖掘HNSC的临床信息和[具体基因名称未给出]表达数据。通过生物信息学分析HNSC肿瘤中[具体基因名称未给出]表达与免疫细胞浸润之间的相关性，并进行基因富集分析。在HNSC细胞系中沉默[具体基因名称未给出]，通过qRT-PCR和蛋白质免疫印迹法验证基因和分子水平的变化。研究人员还通过一系列细胞功能实验验证了其功能表型。结果表明，HNSC中[具体基因名称未给出]的过表达和拷贝数变异与较差的预后相关。此外，[具体基因名称未给出]表达与免疫细胞浸润显著负相关。在HNSC细胞中沉默[具体基因名称未给出]可显著降低细胞迁移率。[具体基因名称未给出]可能通过调节上皮-间质转化影响HNSC的进展。总之，[具体基因名称未给出]是HNSC预后的潜在生物标志物和免疫治疗的潜在靶点。
