Kijsamanmith Kanittha, Srisatayasatien Panisara, Thanindratarn Nichapa, Vichainarong Chanisa, Panyasukum Jirapat
Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
J Dent Sci. 2024 Apr;19(2):846-854. doi: 10.1016/j.jds.2023.09.004. Epub 2023 Sep 9.
BACKGROUND/PURPOSE: Iontophoresis could enhance the delivery of chlorhexidine into oral tissue. This study aimed to determine the effect of 2% chlorhexidine iontophoresis (CHI) on the sealing ability of etch-and-rinse adhesive in human dentin using hydraulic conductance (HD) measurement, scanning electron microscopy and energy dispersive x-ray spectroscopy (SEM-EDS).
Thirty-nine sound dentin specimens were prepared from 39 extracted intact third molars. Thirty specimens were used for HD measurement and randomly divided into 3 equal-sized groups; (1) No chlorhexidine treatment (control), (2) passive chlorhexidine treatment (CHT) and (3) CHI on acid-etched dentin. Each dentin surface was treated with etch-and-rinse adhesive. HD of each specimen was measured before treatment, after immediate bonding and after 14 days. The other 9 specimens were subjected to SEM-EDS analysis of the acid-etched dentin and the dentin treated with CHT and CHI. ANOVA test and Student-Newman-Keuls method were used for statistical analysis.
After bonding, there was no significant difference in percentage decrease of HD among the treatment groups ( > 0.05). After 14 days, CHI and CHT groups had greater percentage decrease of HD than the control ( < 0.001 and = 0.009, respectively). Under SEM-EDS analysis, acid-etched dentin with CHI presented opened dentinal tubule orifices and more chlorhexidine precipitates on dentin than the dentin with CHT, which strongly related to a higher percentage of chloride ions on the CHI dentin surface ( < 0.001).
The use of CHI on acid-etched dentin had a positive effect on dentin sealing ability of etch-and-rinse adhesive.
背景/目的：离子导入法可增强洗必泰向口腔组织的递送。本研究旨在通过液压传导率（HD）测量、扫描电子显微镜和能量色散X射线光谱（SEM-EDS）来确定2%洗必泰离子导入法（CHI）对酸蚀冲洗粘结剂在人牙本质中封闭能力的影响。
从39颗完整拔除的第三磨牙制备39个健康牙本质标本。30个标本用于HD测量，并随机分为3组，每组样本量相等；（1）未用洗必泰处理（对照组），（2）被动洗必泰处理（CHT），（3）酸蚀牙本质上进行CHI处理。每个牙本质表面均用酸蚀冲洗粘结剂处理。在处理前、即刻粘结后和14天后测量每个标本的HD。另外9个标本对酸蚀牙本质以及经CHT和CHI处理的牙本质进行SEM-EDS分析。采用方差分析和Student-Newman-Keuls法进行统计分析。
粘结后，各处理组HD降低百分比无显著差异（P>0.05）。14天后，CHI组和CHT组的HD降低百分比均高于对照组（分别为P<0.001和P = 0.009）。在SEM-EDS分析下，与CHT处理的牙本质相比，CHI处理的酸蚀牙本质呈现开放的牙本质小管口，且牙本质上有更多的洗必泰沉淀，这与CHI处理的牙本质表面更高百分比的氯离子密切相关（P<0.001）。
在酸蚀牙本质上使用CHI对酸蚀冲洗粘结剂的牙本质封闭能力有积极影响。
