Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District,Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006331.
A Gram-stain-negative, rod-shaped, indole-producing, and cellulose-degrading bacterial strain, designated NEAU-G-C5, was isolated from soil collected from a forest in Dali city, Yunnan province, south China. 16S rRNA gene sequence analysis showed that strain NEAU-G-C5 was assigned to the genus and showed high sequence similarities to 12-OD1 (98.32 %) and 6 NM-7 (98.41 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G-C5 formed a lineage related to 12-OD1 and 6 NM-7. The major fatty acids of the strain were C, C ω7, and C cyclo. The respiratory quinone was Q-8. The polar lipid profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. In addition, the average nucleotide identity values between strain NEAU-G-C5 and its reference strains 12-OD1, 6 NM-7, NS9, and TSA1 were 89.7, 88.2, 81.3, and 88.0 %, respectively, and the levels of digital DNA-DNA hybridization between them were found to be 58.5 % (54.9-62.0 %), 53.2 % (49.8-56.7 %), 31.9 % (28.6-35.5 %), and 57.7 % (54.1-61.2 %), respectively, which were lower than the accepted threshold values of 95-96 % and 70 %, respectively. The DNA G+C content of strain NEAU-G-C5 was 66.5 mol%. The strain could produce indoleacetic acid and cellulase. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, we conclude that strain NEAU-G-C5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-G-C5 (=MCCC 1K08668=KCTC 8080).
一株革兰氏阴性、杆状、产吲哚和纤维素降解的细菌菌株，被命名为 NEAU-G-C5，从中国南部云南省大理市森林土壤中分离出来。16S rRNA 基因序列分析表明，菌株 NEAU-G-C5 被归入 属，与 12-OD1（98.32%）和 6-NM-7（98.41%）的序列相似度较高。基于 16S rRNA 基因序列的系统发育分析表明，菌株 NEAU-G-C5 与 12-OD1 和 6-NM-7 形成了一个分支。该菌株的主要脂肪酸为 C、Cω7 和 C 环。呼吸醌为 Q-8。菌株的极性脂谱显示存在双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。此外，菌株 NEAU-G-C5 与其参考菌株 12-OD1、6-NM-7、NS9 和 TSA1 的平均核苷酸同一性值分别为 89.7%、88.2%、81.3%和 88.0%，它们之间的数字 DNA-DNA 杂交水平分别为 58.5%（54.9-62.0%）、53.2%（49.8-56.7%）、31.9%（28.6-35.5%）和 57.7%（54.1-61.2%），均低于 95-96%和 70%的可接受阈值。菌株 NEAU-G-C5 的 DNA G+C 含量为 66.5mol%。该菌株能产生吲哚乙酸和纤维素酶。基于表型、基因型和化学分类特征，我们得出结论，菌株 NEAU-G-C5 代表了 属的一个新种，建议将其命名为 sp. nov.。其模式菌株为 NEAU-G-C5（=MCCC 1K08668=KCTC 8080）。
