Bio-fabrication of thermozyme-based nano-biosensors: their components and present scenario.

An amalgamation of microbiology, biocatalysis, recombinant molecular biology, and nanotechnology is crucial for groundbreaking innovation in developing nano-biomedicines and sensoristics. Enzyme-based nano-biosensor finds prospective applications in various sectors (environmental, pharmaceutical, food, biorefineries). These applications demand reliable catalytic efficiency and functionality of the enzyme under an extreme operational environment for a prolonged period. Over the last few years, bio-fabrication of nano-biosensors in conjunction with thermozymes from thermophilic microbes is being sought after as a viable design. Thermozymes are known for their robustness, are chemically resistant toward organic solvents, possess higher durability for constant use, catalytic ability, and stability at elevated temperatures. Additionally, several other attributes of thermozymes like substrate specificity, selectivity, and sensitivity make them desirable in developing a customized biosensor. In this review, crucial designing aspects of enzyme-based nano-biosensors like enzyme immobilization on an electrode surface, new materials derived from microbial sources (biopolymers based nanocomposites), improvisation measures for sensitivity, and selectivity have been addressed. It also covers microbial biosynthesis of nanomaterials used to develop sensoristic devices and its numerous applications such as wastewater treatment, biorefineries, and diagnostics. The knowledge will pave the way toward creating consistent eco-friendly, economically viable nanostructured-based technologies with broad applicability and exploitation for industrial use in the near future.