Sharapova A V, Semenkov I N, Krechetov P P, Lednev S A, Koroleva T V
Lomonosov Moscow State University, 119991 Moscow, Russia.
Eurasia Soil Sci. 2022;55(2):235-241. doi: 10.1134/S1064229322020119. Epub 2022 Mar 28.
The results of a laboratory experiment modeling the effect of kerosene contamination on the cellulolytic activity of microbiocenosis of the Albic Retisol (Kaluga oblast, Russia) and Arenosol (Kyzylorda oblast, Republic of Kazakhstan) humus horizons are described. Cellulolytic activity is assessed according to the rate of weight loss in linen cloth fragments during the incubation for 0-3, 3-7, and 7-13 months. The intensity of cellulolytic activity in the unpolluted Albic Retisol is higher as compared with the Arenosol, which is determined by low acidity and an elevated content of organic matter and nutrients. The soil pollution with kerosene to 10 g/kg causes a reversible change in cellulolytic activity of both Albic Retisol and Arenosol (Aridic). A high load of kerosene (≥25 g/kg) inhibits cellulolytic activity in both soils over 13 months of observation.
The online version contains supplementary material available at 10.1134/S1064229322020119. Cellulolytic activity of soils in a laboratory experiment: (a) initial state of linen tissue (test object) and (b) linen tissue after three months of incubation. Properties of soil humus horizons. Initial water content in the studied soil samples with due account for the added kerosene. Cellulolytic activity in soils grouped according to kerosene load (rate of mass loss, mg/g soil per day. Significance of differences between cellulolytic activities of contaminated soil samples relative control samples according to the Mann-Whitney U-test. Kerosene content in the studied soil samples at the end of the experiment, % of the initial content.
描述了一项实验室实验的结果，该实验模拟了煤油污染对白浆土（俄罗斯卡卢加州）和红砂土（哈萨克斯坦共和国克孜勒奥尔达州）腐殖质层微生物群落纤维素分解活性的影响。纤维素分解活性根据亚麻布碎片在0 - 3个月、3 - 7个月和7 - 13个月培养期间的失重率进行评估。与红砂土相比，未受污染的白浆土中纤维素分解活性更高，这是由低酸度以及较高的有机质和养分含量决定的。煤油污染至10 g/kg会导致白浆土和红砂土（干旱土）的纤维素分解活性发生可逆变化。在13个月的观察期内，高负荷煤油（≥25 g/kg）会抑制两种土壤中的纤维素分解活性。
在线版本包含可在10.1134/S1064229322020119获取的补充材料。实验室实验中土壤的纤维素分解活性：(a)亚麻组织（测试对象）的初始状态和(b)培养三个月后的亚麻组织。土壤腐殖质层的性质。考虑添加煤油后，所研究土壤样品的初始含水量。根据煤油负荷分组的土壤中的纤维素分解活性（失重率，mg/g土壤/天）。根据曼-惠特尼U检验，污染土壤样品与对照样品纤维素分解活性差异的显著性。实验结束时所研究土壤样品中的煤油含量，占初始含量的百分比。
