藏药红药中的化学成分及其细胞毒活性评价。
Chemical constituents of Rubia tibetica Hook. f. from Tibetan medicine and cytotoxic activity evaluation.
机构信息
Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering and Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing 211189, PR China.
The Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibetan Plateau in Qinghai Province, School of Chemistry and Chemical Engineering, Qinghai Normal university, Xining 810008, China.
出版信息
Fitoterapia. 2024 Jun;175:105961. doi: 10.1016/j.fitote.2024.105961. Epub 2024 Apr 16.
Two unprecedented quinone compounds Rubiaxylm A (1) and Rubiaxylm B (2), along with fifteen known anthraquinones (3-17) were isolated and characterized from the roots of Rubia tibetica in Tibetan medicine. Their structures were identified through comprehensive analyses of 1D/2D NMR as well as HR-ESIMS data. Furthermore, all separated compounds were evaluated for their cytotoxic activity on A549, Caco-2, MDA-MB-231 and Skov-3 cell lines. In particular, compound 2 effectively inhibited MDA-MB-231 cells with an IC value of 8.15 ± 0.20 μM. Subsequently, the anti-tumor mechanism of 2 was investigated by flow cytometry, JC-1 staining, cell scratching and cell colony. These results indicated that compound 2 could inhibit the proliferation of MDA-MB-231 cells by arresting cells in the G1 phase.
从藏药中分离得到两种前所未有的醌类化合物 Rubiaxylm A（1）和 Rubiaxylm B（2），以及 15 种已知的蒽醌类化合物（3-17）。通过 1D/2D NMR 和 HR-ESIMS 数据分析，鉴定了它们的结构。此外，对所有分离得到的化合物进行了 A549、Caco-2、MDA-MB-231 和 Skov-3 细胞系的细胞毒性活性评价。特别是，化合物 2 对 MDA-MB-231 细胞的抑制活性较强，IC 值为 8.15±0.20μM。随后，通过流式细胞术、JC-1 染色、细胞划痕和细胞集落实验，研究了 2 的抗肿瘤机制。结果表明，化合物 2 可以通过将细胞阻滞在 G1 期来抑制 MDA-MB-231 细胞的增殖。
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