Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.
Anticancer Agents Med Chem. 2024;24(3):224-234. doi: 10.2174/0118715206284107231120063630.
The side effects of conventional therapeutics pose a problem for cancer treatment. Recently, combination treatments with natural compounds have attracted attention regarding limiting the side effects of treatment. Oleuropein is a natural polyphenol in olives that has antioxidant and anticancer effects.
This study aimed to investigate the oxidative stress effect of a combination of Paclitaxel, a chemotherapeutic agent, and Oleuropein in the MCF-7 cell line.
The xCELLigence RTCA method was used to determine the cytotoxic effects of Oleuropein and Paclitaxel in the MCF-7 cell line. The Total Oxidant and Total Antioxidant Status were analyzed using a kit. The Oxidative Stress Index was calculated by measuring Total Oxidant and Total Antioxidant states. The levels of superoxide dismutase, reduced glutathione and malondialdehyde, which are oxidative stress markers, were also measured by ELISA assay kit.
As a result of the measurement, IC doses of Oleuropein and Paclitaxel were determined as 230 μM and 7.5 μM, respectively. Different percentages of combination ratios were generated from the obtained IC values. The effect of oxidative stress was investigated at the combination rates of 10%, 20%, 30%, and 40% which were determined to be synergistic. In terms of the combined use of Oleuropein and Paclitaxel on oxidative stress, antioxidant defense increased, and Oxidative Stress Index levels decreased.
These findings demonstrate that the doses administered to the Oleuropein+Paclitaxel combination group were lower than those administered to groups using one agent alone (e.g. Paclitaxel), the results of which reduce the possibility of administering toxic doses.
传统疗法的副作用给癌症治疗带来了问题。最近，天然化合物联合治疗引起了人们的关注，以期限制治疗的副作用。橄榄苦苷是橄榄中的一种天然多酚，具有抗氧化和抗癌作用。
本研究旨在探讨紫杉醇（一种化疗药物）与橄榄苦苷联合应用对 MCF-7 细胞系的氧化应激效应。
采用 xCELLigence RTCA 法测定橄榄苦苷和紫杉醇对 MCF-7 细胞系的细胞毒性作用。采用试剂盒分析总氧化剂和总抗氧化状态。通过测量总氧化剂和总抗氧化状态来计算氧化应激指数。还通过 ELISA 试剂盒测定超氧化物歧化酶、还原型谷胱甘肽和丙二醛等氧化应激标志物的水平。
通过测量，确定橄榄苦苷和紫杉醇的 IC 剂量分别为 230 μM 和 7.5 μM。从获得的 IC 值生成了不同比例的组合比率。在确定为协同作用的 10%、20%、30%和 40%的组合比率下，研究了氧化应激的影响。就橄榄苦苷和紫杉醇联合使用对氧化应激的影响而言，抗氧化防御增加，氧化应激指数水平降低。
这些发现表明，橄榄苦苷+紫杉醇联合组的给药剂量低于单独使用一种药物（如紫杉醇）的组，这降低了给予毒性剂量的可能性。
