Kucukgul Altug, Isgor Mehmet M, Duzguner Vesile, Atabay Meryem N, Kucukgul Azime
Department of Biochemistry, Faculty of Veterinary, Mustafa Kemal University, Hatay, Turkey.
Department of Nursery, High School of Health Sciences, Ardahan University, Ardahan, Turkey.
Antiinflamm Antiallergy Agents Med Chem. 2020;19(1):74-84. doi: 10.2174/1871523018666190201145824.
Persistent oxidative stress can lead to chronic inflammation and mediate most chronic diseases including neurological disorders. Oleuropein has been shown to be a potent antioxidant molecule in olive oil leaf having antioxidative properties.
The aim of this study was to investigate the protective effects of oleuropein against oxidative stress in human glioblastoma cells.
Human glioblastoma cells (U87) were pretreated with oleuropein (OP) essential oil 10 µM. After 30 minutes, 100 µM H2O2 was added to the cells for three hours. Cell survival was quantified by colorimetric MTT assay. Glutathione level, total oxidant capacity, total antioxidant capacity and nitric oxide levels were determined by using specific spectrophotometric methods. The relative gene expression level of iNOS was performed by qRT-PCR method.
According to viability results, the effective concentration of H2O2 (100µM) significantly decreased cell viability and oleuropein pretreatment significantly prevented the cell losses. Oleuropein regenerated total antioxidant capacity and glutathione levels decreased by H2O2 exposure. In addition, nitric oxide and total oxidant capacity levels were also decreased after administration of oleuropein in treated cells.
Oleuropein was found to have potent antioxidative properties in human glioblastoma cells. However, further studies and validations are needed in order to understand the exact neuroprotective mechanism of oleuropein.
持续的氧化应激可导致慢性炎症，并介导包括神经疾病在内的大多数慢性疾病。橄榄苦苷已被证明是橄榄油叶中一种具有抗氧化特性的强效抗氧化分子。
本研究旨在探讨橄榄苦苷对人胶质母细胞瘤细胞氧化应激的保护作用。
人胶质母细胞瘤细胞（U87）用10 μM橄榄苦苷（OP）精油预处理。30分钟后，向细胞中加入100 μM过氧化氢，持续3小时。通过比色MTT法对细胞存活率进行定量。采用特定的分光光度法测定谷胱甘肽水平、总氧化剂能力、总抗氧化能力和一氧化氮水平。通过qRT-PCR法检测诱导型一氧化氮合酶（iNOS）的相对基因表达水平。
根据活力结果，过氧化氢（100 μM）的有效浓度显著降低细胞活力，而橄榄苦苷预处理显著防止细胞损失。橄榄苦苷使总抗氧化能力得以恢复，且过氧化氢暴露导致的谷胱甘肽水平降低。此外，在处理的细胞中给予橄榄苦苷后，一氧化氮和总氧化剂能力水平也降低。
发现橄榄苦苷在人胶质母细胞瘤细胞中具有强大的抗氧化特性。然而，为了了解橄榄苦苷确切的神经保护机制，还需要进一步的研究和验证。
