Whitaker Eugene Joseph, Shah Leena N, Miloradovic Ivan R, Yesilsoy Cemil J, Badve Shloka A
Department of Restorative Dentistry, Temple University School of Dentistry, Philadelphia, PA, USA.
Department of Endodontology, Temple University School of Dentistry, Philadelphia, PA, USA.
J Conserv Dent Endod. 2025 Mar;28(3):253-257. doi: 10.4103/JCDE.JCDE_859_24. Epub 2025 Mar 3.
Loop-mediated isothermal amplification (LAMP) may be used in the future to detect infecting microorganisms. LAMP assays exist for the endodontic pathogens and , but not yet for
To develop a LAMP assay for detecting .
It was an benchtop study.
The National Center for Biotechnology Information GenBank Basic Local Alignment Search Tool was used to identify a segment of the dipeptidyl peptidase 11 (DPP11) gene unique to . A primer design tool was used to generate six primers required for developing the LAMP assay. WarmStart Colorimetric LAMP 2X Master Mix was used to evaluate the LAMP assay, using purified DNA as a control.
Statistical parameters for sensitivity and specificity.
The assay was performed in triplicate on pure DNA from and and on the DNA that was extracted from , , , and cells and diluted two-fold from 1/2 to 1/256. Assays for the diluted samples were performed in triplicate, and the contingency tables indicated the LAMP assay to be 82% sensitive and 90% specific for .
LAMP assay could be a highly sensitive and specific chairside detection method for .
环介导等温扩增技术（LAMP）未来可能用于检测感染性微生物。目前已有针对牙髓病病原体[具体病原体1]和[具体病原体2]的LAMP检测方法，但尚未有针对[目标病原体]的检测方法。
开发一种用于检测[目标病原体]的LAMP检测方法。
这是一项实验室台面研究。
利用美国国立生物技术信息中心的GenBank基本局部比对搜索工具，确定[目标病原体]特有的二肽基肽酶11（DPP11）基因片段。使用引物设计工具生成开发LAMP检测方法所需的六种引物。采用热启动比色LAMP 2X预混液，以纯化的[目标病原体]DNA作为对照，对LAMP检测方法进行评估。
敏感性和特异性的统计参数。
该检测方法对[目标病原体]的纯DNA以及从[具体细胞类型1]、[具体细胞类型2]、[具体细胞类型3]和[具体细胞类型4]细胞中提取并从1/2到1/256进行两倍稀释的DNA进行了三次重复检测。对稀释样本的检测进行了三次重复，列联表显示LAMP检测方法对[目标病原体]的敏感性为82%，特异性为90%。
LAMP检测方法可能是一种用于[目标病原体]的高敏感性和特异性的椅旁检测方法。
