Bigay J, Deterre P, Pfister C, Chabre M
FEBS Lett. 1985 Oct 28;191(2):181-5. doi: 10.1016/0014-5793(85)80004-1.
Fluoride activation of the cGMP cascade of vision requires the presence of aluminum, and is shown to be mediated by the binding of one A1F-4 to the GDP/GTP-binding subunit of transducin. The presence of GDP in the site is required: A1F-4 is ineffective when the site is empty or when GDP beta S is substituted for GDP. This sensitivity to the sulfur of GDP beta S suggests that A1F-4 is in contact with the GDP. Striking structural similarities between A1F-4 and PO3-4 lead us to propose that A1F-4 mimics the role of the gamma-phosphate of GTP.
氟化物对视觉cGMP级联反应的激活需要铝的存在，并且已表明是由一个AlF₄⁻与转导素的GDP/GTP结合亚基结合介导的。该位点需要有GDP存在：当该位点为空或用GDP-β-S替代GDP时，AlF₄⁻无效。对GDP-β-S中硫的这种敏感性表明AlF₄⁻与GDP接触。AlF₄⁻和PO₄³⁻之间显著的结构相似性使我们提出AlF₄⁻模拟了GTP的γ-磷酸基团的作用。
