State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 210023, Nanjing, China.
Department of Clinical Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University, 210008, Nanjing, China.
Nat Commun. 2024 Apr 18;15(1):3343. doi: 10.1038/s41467-024-47710-w.
Pathogenic gut microbiota is responsible for a few debilitating gastrointestinal diseases. While the host immune cells do produce extracellular vesicles to counteract some deleterious effects of the microbiota, the extracellular vesicles are of insufficient doses and at unreliable exposure times. Here we use mechanical stimulation of hydrogel-embedded macrophage in a bioelectronic controller that on demand boost production of up to 20 times of therapeutic extracellular vesicles to ameliorate the microbes' deleterious effects in vivo. Our miniaturized wireless bioelectronic system termed inducible mechanical activation for in-situ and sustainable generating extracellular vesicles (iMASSAGE), leverages on wireless electronics and responsive hydrogel to impose mechanical forces on macrophages to produce extracellular vesicles that rectify gut microbiome dysbiosis and ameliorate colitis. This in vivo controllable extracellular vesicles-produced system holds promise as platform to treat various other diseases.
致病的肠道微生物群是一些使人衰弱的胃肠道疾病的罪魁祸首。虽然宿主免疫细胞确实会产生细胞外囊泡来抵消微生物群的一些有害影响，但细胞外囊泡的剂量不足，且暴露时间不可靠。在这里，我们使用生物电子控制器中嵌入水凝胶的巨噬细胞的机械刺激，按需提高多达 20 倍的治疗性细胞外囊泡的产量，以改善体内微生物的有害影响。我们的微型化无线生物电子系统称为诱导机械激活原位和可持续产生细胞外囊泡（iMASSAGE），利用无线电子和响应性水凝胶对巨噬细胞施加机械力，产生细胞外囊泡，纠正肠道微生物组失调并改善结肠炎。这种可在体内控制的细胞外囊泡产生系统有望成为治疗各种其他疾病的平台。
