Jafar Mohammed, Ahmad Khan Mohd Sajjad, Akbar Mohammad Jamal, AlSaihaty Hadi Saleem, Alasmari Sultan Saad
Department of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 34212, Saudi Arabia.
Department of Basic Sciences, Deanship of Preparatory Year and Supporting Studies, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 34212, Saudi Arabia.
Heliyon. 2024 Apr 8;10(8):e29246. doi: 10.1016/j.heliyon.2024.e29246. eCollection 2024 Apr 30.
Thyme oil (TO) is a valuable essential oil believed to possess a variety of bioactivities, including antibacterial, anticancer, and antioxidant properties. These attributes grant TO the excellent capability to treat a wide range of diseases, particularly the effective eradication of infection in the stomach. However, its practical use is limited by its low stability under atmospheric conditions. Our current research aims to encapsulate TO in eudragit (EGT) microsponges to enhance its stability and improve its effectiveness against . The TO microsponges were prepared using EGT as a polymer, polysorbate 80 as a stabilizer, and dichloromethane (DCM) as a solvent via the quasi-emulsion solvent evaporation method. The product yield, particle size, surface morphology, entrapment efficiency, drug-polymer interaction, in-vitro floating, and in-vitro drug release of the microsponges were evaluated. The most promising microsponge was tested against ATCC 43504 strains. The results showed that the microsponges exhibited a high product yield (ranging from 41 % ± 0.75-81.27 % ± 1.13), excellent entrapment efficiency (ranging from 63.01 % ± 0.79-88.64 % ± 0.98), prolonged in-vitro floating time (more than 12 h) and sustained in-vitro drug release for 18 h (81.53 %). Scanning electron microscopy results indicated that the microsponges were spherical in shape with a spongy surface. The average particle size of the selected microsponges was determined to be 49.79 ± 1.4 μm, and their average pore size was measured to be 0.81 ± 0.14 μm. DSC study results revealed that TO was physically entrapped in the microsponges. In-vitro - activity studies demonstrated that TO in microsponge was more effective against than pure TO. In conclusion, the developed microsponges containing thyme oil provide a promising alternative for the efficient targeting and eradication of infection.
百里香油（TO）是一种珍贵的精油，据信具有多种生物活性，包括抗菌、抗癌和抗氧化特性。这些特性使百里香油具有出色的能力来治疗多种疾病，特别是有效根除胃部感染。然而，其实际应用受到大气条件下稳定性低的限制。我们目前的研究旨在将百里香油包封在丙烯酸树脂（EGT）微海绵中，以提高其稳定性并增强其对抗[具体病菌]的效果。通过准乳液溶剂蒸发法，以EGT为聚合物、聚山梨酯80为稳定剂、二氯甲烷（DCM）为溶剂制备了百里香油微海绵。对微海绵的产品收率、粒径、表面形态、包封率、药物 - 聚合物相互作用、体外漂浮性和体外药物释放进行了评估。对最有前景的微海绵针对[具体病菌]ATCC 43504菌株进行了测试。结果表明，微海绵具有较高的产品收率（范围为41%±0.75 - 81.27%±1.13）、出色的包封率（范围为63.01%±0.79 - 88.64%±0.98）、延长的体外漂浮时间（超过12小时）以及18小时的持续体外药物释放（81.53%）。扫描电子显微镜结果表明，微海绵呈球形，表面为海绵状。所选微海绵的平均粒径确定为49.79±1.4μm，平均孔径测量为0.81±0.14μm。差示扫描量热法研究结果表明，百里香油被物理包封在微海绵中。体外[抗菌]活性研究表明，微海绵中的百里香油比纯百里香油对[具体病菌]更有效。总之，所开发的含百里香油微海绵为有效靶向和根除[具体病菌]感染提供了一种有前景的替代方法。
