Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India.
Department of Pharmaceutical Sciences, Maharishi Dayanand University, Rohtak, 1240001, Haryana, India.
J Food Drug Anal. 2019 Jan;27(1):60-70. doi: 10.1016/j.jfda.2018.07.006. Epub 2018 Aug 16.
Babchi essential oil (BEO) is a valuable essential oil reported to possess a variety of biological activities such as antitumor, anti inflammatory, immunomodulatory, antioxidant, antifungal and antibacterial properties. Due to its anti-microbial properties, this oil possesses an immense potential for the treatment of dermatological disorders. Further, it has minimal tendency to develop resistance, a common issue with most of the antibiotics. However, its highly viscous nature and poor stability in the presence of light, air and high temperature, limits its practical applications. To surmount these issues, this research aims to encapsulate BEO in ethyl cellulose (EC) microsponges for enhanced stability, antibacterial effect and decreased dermal toxicity. The quasi emulsion solvent evaporation technique was used for fabrication of the BEO microsponges employing EC as polymer, polyvinyl alcohol (PVA) as stabilizer and dichloro methane (DCM) as solvent. The effect of formulation variables such as the amount of EC and PVA were also investigated. The prepared microformulations were evaluated for production yield, encapsulation efficiency, particle size and in vitro release. In vitro cytotoxicity was also checked to assess dermal safety of BEO microsponges. Results revealed that all the dispersions were in micro size range (20.44 ± 3.13 μm to 41.75 ± 3.65 μm), with good encapsulation efficiency (87.70 ± 1.20% of F2) and controlled release profile (cumulative drug release 73.34 ± 1.76%). Field emission scanning electron microscopy results showed that the microsponges possessed a spherical uniform shape with a spongy structure. Results of cytotoxicity study indicated that the prepared microsponges were safer on dermal cells in comparison to pure BEO. The optimized formulation was also evaluated for in vitro antimicrobial assay against dermal bacteria like Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, which confirmed their enhanced antibacterial activity. Furthermore, the results of photostability and stability analysis indicated improved stability of BEO loaded microsponges. Hence, encapsulation of BEO in microsponges resulted in efficacious carrier system in terms of stability as well as safety of this essential oil alongwith handling benefits.
巴茇精油（BEO）是一种有价值的精油，据报道具有多种生物活性，如抗肿瘤、抗炎、免疫调节、抗氧化、抗真菌和抗菌特性。由于其抗菌特性，这种油具有治疗皮肤病的巨大潜力。此外，它几乎没有产生抗药性的倾向，这是大多数抗生素的一个常见问题。然而，由于其高粘性和在光、空气和高温下的不稳定性，限制了其实际应用。为了克服这些问题，本研究旨在将 BEO 包封在乙基纤维素（EC）微球中，以提高其稳定性、抗菌效果和降低皮肤毒性。准乳液溶剂蒸发技术用于制备 BEO 微球，采用 EC 作为聚合物、聚乙烯醇（PVA）作为稳定剂和二氯甲烷（DCM）作为溶剂。还研究了制剂变量（如 EC 和 PVA 的用量）的影响。对制备的微制剂进行了产率、包封效率、粒径和体外释放的评价。还检查了体外细胞毒性，以评估 BEO 微球的皮肤安全性。结果表明，所有分散体均处于微尺寸范围（20.44±3.13μm 至 41.75±3.65μm），具有良好的包封效率（F2 的 87.70±1.20%）和控制释放特性（累积药物释放 73.34±1.76%）。场发射扫描电子显微镜结果表明，微球具有球形均匀形状和海绵状结构。细胞毒性研究结果表明，与纯 BEO 相比，制备的微球对皮肤细胞更安全。还对优化的配方进行了体外抗皮肤细菌（如金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌）的抗菌试验评估，证实了其增强的抗菌活性。此外，光稳定性和稳定性分析结果表明，负载 BEO 的微球的稳定性得到了提高。因此，将 BEO 包封在微球中，得到了一种有效的载体制剂，在这种精油的稳定性和安全性方面具有良好的效果，同时还具有处理优势。
